The University of Southampton
University of Southampton Institutional Repository

Synthesis and evaluation of fluorescent probes for the detection of calpain activity

Mittoo, Stifun, Sundstrom, Lars E. and Bradley, Mark (2003) Synthesis and evaluation of fluorescent probes for the detection of calpain activity Analytical Biochemistry, 319, (2), pp. 234-238. (doi:10.1016/S0003-2697(03)00324-5).

Record type: Article


Two new probes for the detection of calpain I activity based on fluorescence resonance energy transfer technology have been synthesized and evaluated. The probes incorporated the cleavage site present in ?-spectrin, a naturally occurring substrate of calpain I. The design of the internally quenched substrates is such that the calpain-sensitive bond of the peptides (between the Tyr-Gly residues) is located centrally between the donor and the quencher chromophores. The calpain assay protocol is capable of detecting enzymatic activity in the nanomolar region.

Full text not available from this repository.

More information

Published date: 2003
Keywords: calpain i, fret, fluorescence, proteolytic activity


Local EPrints ID: 27662
ISSN: 0003-2697
PURE UUID: f7ea0a30-c401-4f70-9704-73eef4c0aecb

Catalogue record

Date deposited: 28 Apr 2006
Last modified: 17 Jul 2017 16:04

Export record



Author: Stifun Mittoo
Author: Lars E. Sundstrom
Author: Mark Bradley

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton:

ePrints Soton supports OAI 2.0 with a base URL of

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.