The University of Southampton
University of Southampton Institutional Repository

Neuropil and neuronal changes in hippocampal NADPH-diaphaorase histochemistry in the ME7 model of murine prion disease

Picanço-Diniz, CW., Boche, D., Gomes-Leal, W., Perry, VH. and Cunningham, C. (2004) Neuropil and neuronal changes in hippocampal NADPH-diaphaorase histochemistry in the ME7 model of murine prion disease Neuropathology and Applied Neurobiology, 30, (3), pp. 292-303. (doi:10.1111/j.1365-2990.2004.00537.x).

Record type: Article

Abstract

Nitric oxide (NO) has been implicated in neurotoxicity and cerebral blood flow changes in chronic neurodegeneration, but its activity in the mammalian prion diseases has not been studied in detail. Nicotine adenine dinucleotide phosphate (NADPH)-diaphorase (NADPH-d) histochemistry is a simple and robust histochemical procedure that allows localization of the tissue distribution of NO synthases. The aim of the present study is to assess whether NADPH-d histochemical activity is altered in the hippocampus in the ME7 model of prion disease in C57BL/6J mice. At early and late stages after the initiation of the disease we assessed features of the NADPH-d positive cells and the neuropil histochemical activity in CA1 and dentate gyrus using densitometric analysis. In C57BL/6J mice 13 weeks postinjection of the prion agent ME7, when behavioural changes first become apparent, neuropil NADPH-d histochemical staining increases, whereas at late stages it decreases dramatically. Both type I and type II NADPH-d positive cells were found to survive throughout the hippocampal formation into the late stages of the disease, but diaphorase activity was reduced in dendritic branches and abnormal varicosities were present in both dendritic and axonal processes of NADPH-d positive type I cells. The pathophysiological implications of the results remain to be investigated but both blood flow alteration and NO neurotoxicity may be features of the disease

Full text not available from this repository.

More information

Published date: June 2004
Keywords: blood flow, neurodegeneration, nitric oxide, peroxynitrite, scrapie

Identifiers

Local EPrints ID: 27685
URI: http://eprints.soton.ac.uk/id/eprint/27685
ISSN: 0305-1846
PURE UUID: 89e13436-8231-4ae8-831d-14a2f769a9a9
ORCID for D. Boche: ORCID iD orcid.org/0000-0002-5884-130X

Catalogue record

Date deposited: 28 Apr 2006
Last modified: 17 Jul 2017 16:04

Export record

Altmetrics

Contributors

Author: CW. Picanço-Diniz
Author: D. Boche ORCID iD
Author: W. Gomes-Leal
Author: VH. Perry
Author: C. Cunningham

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×