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The expression of the developmentally regulated proto-oncogene Pax-3 is modulated by N-Myc

Record type: Article

N-Myc is a member of the Myc family of transcription factors that have been shown to play a pivotal role in cell proliferation and differentiation. In this report, we have investigated the relationship between N-Myc and the developmental control gene Pax-3. Using transient transfection assays, we show that the Pax-3 promoter is activated by both N-Myc-Max and c-Myc-Max. Moreover, we show that Myc regulation of Pax-3 promoter activity is dependent upon a noncanonical E box site in the 5' promoter region of Pax-3. In addition, we show that ectopic expression of both N-Myc and c-Myc leads to increased expression of Pax-3 mRNA. Furthermore, we show that Pax-3 mRNA expression is cell cycle-regulated and that the 5' promoter region of Pax-3 (bp - 1578 to +56) can direct cell cycle-dependent gene expression with kinetics similar to that of the endogenous transcript. Site-directed mutagenesis of the E box site within the Pax-3 promoter significantly altered the pattern of expression through the cell cycle. These results suggest that the Myc family of transcription factors may modulate Pax-3 expression in vivo.

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Citation

Harris, Robert G., White, Edward, Phillips, Emma S. and Lillycrop, Karen A. (2002) The expression of the developmentally regulated proto-oncogene Pax-3 is modulated by N-Myc The Journal of Biological Chemistry, 277, (38), pp. 34815-34825. (doi:10.1074/jbc.M109609200).

More information

Submitted date: 4 October 2001
Published date: 2 July 2002

Identifiers

Local EPrints ID: 28824
URI: http://eprints.soton.ac.uk/id/eprint/28824
ISSN: 0021-9258
PURE UUID: a3146151-c905-42ad-bc60-2c7f924b422e
ORCID for Karen A. Lillycrop: ORCID iD orcid.org/0000-0001-7350-5489

Catalogue record

Date deposited: 08 May 2006
Last modified: 17 Jul 2017 15:59

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Contributors

Author: Robert G. Harris
Author: Edward White
Author: Emma S. Phillips

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