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Heterogeneous proliferation within engineered cartilaginous tissue: the role of oxygen tension

Heterogeneous proliferation within engineered cartilaginous tissue: the role of oxygen tension
Heterogeneous proliferation within engineered cartilaginous tissue: the role of oxygen tension
This article investigates heterogeneous proliferation within a seeded three-dimensional scaffold structure with the purpose of improving protocols for engineered tissue growth. A simple mathematical model is developed to examine the very strong interaction between evolving oxygen profiles and cell distributions within cartilaginous constructs. A comparison between predictions based on the model and experimental evidence is given for both spatial and temporal evolution of the oxygen tension and cell number density, showing that behaviour for the first 14 days can be explained well by the mathematical model.
The dependency of the cellular proliferation rate on the oxygen tension is examined and shown to be similar in size to previous work but linear in form. The results show that cell-scaffold constructs that rely solely on diffusion for their supply of nutrients will inevitably produce proliferation-dominated regions near the outer edge of the scaffold in situations when the cell number density and oxygen consumption rate exceed a critical level. Possible strategies for reducing such non-uniform proliferation, including the conventional methods of enhancing oxygen transport, are outlined based on the model predictions.
tissue engineering, mathematical modelling, oxygen gradient, scaffold, seeding
0006-3592
607-615
Lewis, Miranda C.
e2341eb3-5938-495a-b80c-f9fa0e90f1ee
MacArthur, Ben D.
2c0476e7-5d3e-4064-81bb-104e8e88bb6b
Malda, Jos
3dfabcc1-e338-4a21-b1cc-40497d72d361
Pettet, Graeme
2f9281f9-7d2d-404d-a6a2-b8fc50e9b1c5
Please, Colin P.
118dffe7-4b38-4787-a972-9feec535839e
Lewis, Miranda C.
e2341eb3-5938-495a-b80c-f9fa0e90f1ee
MacArthur, Ben D.
2c0476e7-5d3e-4064-81bb-104e8e88bb6b
Malda, Jos
3dfabcc1-e338-4a21-b1cc-40497d72d361
Pettet, Graeme
2f9281f9-7d2d-404d-a6a2-b8fc50e9b1c5
Please, Colin P.
118dffe7-4b38-4787-a972-9feec535839e

Lewis, Miranda C., MacArthur, Ben D., Malda, Jos, Pettet, Graeme and Please, Colin P. (2005) Heterogeneous proliferation within engineered cartilaginous tissue: the role of oxygen tension. Biotechnology and Bioengineering, 91 (5), 607-615. (doi:10.1002/bit.20508).

Record type: Article

Abstract

This article investigates heterogeneous proliferation within a seeded three-dimensional scaffold structure with the purpose of improving protocols for engineered tissue growth. A simple mathematical model is developed to examine the very strong interaction between evolving oxygen profiles and cell distributions within cartilaginous constructs. A comparison between predictions based on the model and experimental evidence is given for both spatial and temporal evolution of the oxygen tension and cell number density, showing that behaviour for the first 14 days can be explained well by the mathematical model.
The dependency of the cellular proliferation rate on the oxygen tension is examined and shown to be similar in size to previous work but linear in form. The results show that cell-scaffold constructs that rely solely on diffusion for their supply of nutrients will inevitably produce proliferation-dominated regions near the outer edge of the scaffold in situations when the cell number density and oxygen consumption rate exceed a critical level. Possible strategies for reducing such non-uniform proliferation, including the conventional methods of enhancing oxygen transport, are outlined based on the model predictions.

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More information

Published date: 2005
Keywords: tissue engineering, mathematical modelling, oxygen gradient, scaffold, seeding

Identifiers

Local EPrints ID: 29271
URI: http://eprints.soton.ac.uk/id/eprint/29271
ISSN: 0006-3592
PURE UUID: bd875e0c-3c6c-498a-b181-0051d7ffc459

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Date deposited: 11 May 2006
Last modified: 09 Jan 2022 01:22

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Contributors

Author: Miranda C. Lewis
Author: Jos Malda
Author: Graeme Pettet
Author: Colin P. Please

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