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The development of decision limits for the implementation of the GH-2000 detection methodology using current commercial insulin-like growth factor-I and amino-terminal pro-peptide of type III collagen assays

The development of decision limits for the implementation of the GH-2000 detection methodology using current commercial insulin-like growth factor-I and amino-terminal pro-peptide of type III collagen assays
The development of decision limits for the implementation of the GH-2000 detection methodology using current commercial insulin-like growth factor-I and amino-terminal pro-peptide of type III collagen assays
Background: The GH-2000 project developed a method for detecting GH misuse based on the measurement of insulin-like growth factor-I (IGF-I) and the amino-terminal pro-peptide of type III collagen (P-III-NP). The objective
of this study was to develop decision limits for the GH-2000 score to detect GH misuse in elite athletes using two currently available commercial assays for each analyte.

Study design: Subjects: 404 male (mean age 23.9 yrs, range 12–37 yrs) and 94 female elite athletes (mean age 24.5 yrs, range 18–34 yrs) participated. Blood samples were collected according to World Anti-Doping Agency (WADA) guidelines at various sporting events including 238 samples collected as part of the UK Anti- Doping Testing Programme.

Laboratory analysis: IGF-I was measured by Siemens Immulite IGF-I assay and Immunotech A15729 IGF-IIRMA. P-III-NP was measured by RIA-gnost P-III-P and the UniQ™ PIIINP RIA.

Statistical analysis: The GH-2000 score decision limits were developed through the analysis of the elite athlete samples.

Results: For males and females separately, the distributions of GH-2000 scores were consistent with Normal distributions. Using a specificity of 99.99% new decision limits were determined which included an allowance for uncertainty associated with calculations based on a finite sample size. One outlier was identified with results incompatible with normal physiology and tested positive with the current isoform GH test.

Conclusions: We have developed decision limits using currently available commercial assays to measure IGF-I and P-III-NP in elite athletes. This should allow the introduction of a test for GH misuse based on the measurement of these GH sensitive biomarkers.
growth hormone, doping, test, insulin-like growth factor-I (IGF-I), amino-terminal pro-peptide of type III collagen (P-III-NP)
1096-6374
53-58
Erotokritou-Mulligan, Ioulietta
8bb8720a-eda7-4ad4-b969-0b35c5e5226c
Guha, Nishan
38212040-a65e-4874-a60a-21f0ae3bdf09
Stow, Michael
435d1223-65f5-4186-9d5a-04501bb03052
Bassett, E. Eryl
fefd7f16-2045-4700-adaa-7cc6049933a4
Bartlett, Christiaan
ed9cba27-96ef-419e-824b-fabab5c7cba8
Cowan, David A.
22bdafa8-cee8-481a-97d5-2687fcf325ca
Sonksen, Peter H.
b3cf05ef-65c2-4e9b-8d95-b7c3aaf532cd
Holt, Richard I. G.
d54202e1-fcf6-4a17-a320-9f32d7024393
Erotokritou-Mulligan, Ioulietta
8bb8720a-eda7-4ad4-b969-0b35c5e5226c
Guha, Nishan
38212040-a65e-4874-a60a-21f0ae3bdf09
Stow, Michael
435d1223-65f5-4186-9d5a-04501bb03052
Bassett, E. Eryl
fefd7f16-2045-4700-adaa-7cc6049933a4
Bartlett, Christiaan
ed9cba27-96ef-419e-824b-fabab5c7cba8
Cowan, David A.
22bdafa8-cee8-481a-97d5-2687fcf325ca
Sonksen, Peter H.
b3cf05ef-65c2-4e9b-8d95-b7c3aaf532cd
Holt, Richard I. G.
d54202e1-fcf6-4a17-a320-9f32d7024393

Erotokritou-Mulligan, Ioulietta, Guha, Nishan, Stow, Michael, Bassett, E. Eryl, Bartlett, Christiaan, Cowan, David A., Sonksen, Peter H. and Holt, Richard I. G. (2012) The development of decision limits for the implementation of the GH-2000 detection methodology using current commercial insulin-like growth factor-I and amino-terminal pro-peptide of type III collagen assays. Growth Hormone and IGF Research, 22 (2), 53-58. (doi:10.1016/j.ghir.2011.12.005). (PMID:22305721)

Record type: Article

Abstract

Background: The GH-2000 project developed a method for detecting GH misuse based on the measurement of insulin-like growth factor-I (IGF-I) and the amino-terminal pro-peptide of type III collagen (P-III-NP). The objective
of this study was to develop decision limits for the GH-2000 score to detect GH misuse in elite athletes using two currently available commercial assays for each analyte.

Study design: Subjects: 404 male (mean age 23.9 yrs, range 12–37 yrs) and 94 female elite athletes (mean age 24.5 yrs, range 18–34 yrs) participated. Blood samples were collected according to World Anti-Doping Agency (WADA) guidelines at various sporting events including 238 samples collected as part of the UK Anti- Doping Testing Programme.

Laboratory analysis: IGF-I was measured by Siemens Immulite IGF-I assay and Immunotech A15729 IGF-IIRMA. P-III-NP was measured by RIA-gnost P-III-P and the UniQ™ PIIINP RIA.

Statistical analysis: The GH-2000 score decision limits were developed through the analysis of the elite athlete samples.

Results: For males and females separately, the distributions of GH-2000 scores were consistent with Normal distributions. Using a specificity of 99.99% new decision limits were determined which included an allowance for uncertainty associated with calculations based on a finite sample size. One outlier was identified with results incompatible with normal physiology and tested positive with the current isoform GH test.

Conclusions: We have developed decision limits using currently available commercial assays to measure IGF-I and P-III-NP in elite athletes. This should allow the introduction of a test for GH misuse based on the measurement of these GH sensitive biomarkers.

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More information

Published date: April 2012
Keywords: growth hormone, doping, test, insulin-like growth factor-I (IGF-I), amino-terminal pro-peptide of type III collagen (P-III-NP)
Organisations: Human Development & Health

Identifiers

Local EPrints ID: 301062
URI: http://eprints.soton.ac.uk/id/eprint/301062
ISSN: 1096-6374
PURE UUID: d3e3b732-9ad9-4b1e-a860-591b1b0d5b02
ORCID for Richard I. G. Holt: ORCID iD orcid.org/0000-0001-8911-6744

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Date deposited: 28 Feb 2012 12:42
Last modified: 15 Mar 2024 03:08

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Contributors

Author: Ioulietta Erotokritou-Mulligan
Author: Nishan Guha
Author: Michael Stow
Author: E. Eryl Bassett
Author: Christiaan Bartlett
Author: David A. Cowan
Author: Peter H. Sonksen

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