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Nutrient shock and incubation atmosphere influence recovery of culturable Helicobacter pylori from water

Nutrient shock and incubation atmosphere influence recovery of culturable Helicobacter pylori from water
Nutrient shock and incubation atmosphere influence recovery of culturable Helicobacter pylori from water
Three different media—Columbia agar, Wilkins-Chalgren agar, and Helicobacter pylori special peptone agar—were prepared in a diluted version and compared to the standard medium formulation in order to study a possible nutrient shock effect observed when recovering H. pylori from water by counting the number of CFU. This same parameter was subsequently used to evaluate the influence of the incubation atmosphere by using a modular atmosphere-controlled system to provide different atmospheres and by employing an established gas generation kit as a control. Both a low nutrient content of the media and a rapidly achieved microaerophilic incubation atmosphere proved to increase the numbers of environment-stressed H. pylori organisms recovered. An atmosphere of 5% CO2, 5% O2, and 3% H2 is recommended, although other atmospheres with a low oxygen concentration are also acceptable. Besides highlighting and assessing the importance of several factors in the culturability of H. pylori, this paper demonstrates the potential ability to develop an optimized technique for recovery of this pathogen from water.
0099-2240
490-493
Azevedo, N.F.
c90d7c41-e45a-404d-9472-9d0b411448e7
Pacheco, A.P.
58f03fb4-176d-40f7-bca7-458e60729e64
Keevil, C.W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
Vieira, M.J.
b13909e0-b086-49fc-b589-3fb9d2a02577
Azevedo, N.F.
c90d7c41-e45a-404d-9472-9d0b411448e7
Pacheco, A.P.
58f03fb4-176d-40f7-bca7-458e60729e64
Keevil, C.W.
cb7de0a7-ce33-4cfa-af52-07f99e5650eb
Vieira, M.J.
b13909e0-b086-49fc-b589-3fb9d2a02577

Azevedo, N.F., Pacheco, A.P., Keevil, C.W. and Vieira, M.J. (2004) Nutrient shock and incubation atmosphere influence recovery of culturable Helicobacter pylori from water. Applied and Environmental Microbiology, 70 (1), 490-493. (doi:10.1128/AEM.70.1.490-493.2004).

Record type: Article

Abstract

Three different media—Columbia agar, Wilkins-Chalgren agar, and Helicobacter pylori special peptone agar—were prepared in a diluted version and compared to the standard medium formulation in order to study a possible nutrient shock effect observed when recovering H. pylori from water by counting the number of CFU. This same parameter was subsequently used to evaluate the influence of the incubation atmosphere by using a modular atmosphere-controlled system to provide different atmospheres and by employing an established gas generation kit as a control. Both a low nutrient content of the media and a rapidly achieved microaerophilic incubation atmosphere proved to increase the numbers of environment-stressed H. pylori organisms recovered. An atmosphere of 5% CO2, 5% O2, and 3% H2 is recommended, although other atmospheres with a low oxygen concentration are also acceptable. Besides highlighting and assessing the importance of several factors in the culturability of H. pylori, this paper demonstrates the potential ability to develop an optimized technique for recovery of this pathogen from water.

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More information

Submitted date: 20 June 2003
Published date: 2004

Identifiers

Local EPrints ID: 30164
URI: http://eprints.soton.ac.uk/id/eprint/30164
ISSN: 0099-2240
PURE UUID: 752e6847-00f6-4abc-ac41-251e7dc8564d
ORCID for C.W. Keevil: ORCID iD orcid.org/0000-0003-1917-7706

Catalogue record

Date deposited: 11 May 2006
Last modified: 18 Feb 2021 16:58

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