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Nutrient shock and incubation atmosphere influence recovery of culturable Helicobacter pylori from water

Azevedo, N.F., Pacheco, A.P., Keevil, C.W. and Vieira, M.J. (2004) Nutrient shock and incubation atmosphere influence recovery of culturable Helicobacter pylori from water Applied and Environmental Microbiology, 70, (1), pp. 490-493. (doi:10.1128/AEM.70.1.490-493.2004).

Record type: Article


Three different media—Columbia agar, Wilkins-Chalgren agar, and Helicobacter pylori special peptone agar—were prepared in a diluted version and compared to the standard medium formulation in order to study a possible nutrient shock effect observed when recovering H. pylori from water by counting the number of CFU. This same parameter was subsequently used to evaluate the influence of the incubation atmosphere by using a modular atmosphere-controlled system to provide different atmospheres and by employing an established gas generation kit as a control. Both a low nutrient content of the media and a rapidly achieved microaerophilic incubation atmosphere proved to increase the numbers of environment-stressed H. pylori organisms recovered. An atmosphere of 5% CO2, 5% O2, and 3% H2 is recommended, although other atmospheres with a low oxygen concentration are also acceptable. Besides highlighting and assessing the importance of several factors in the culturability of H. pylori, this paper demonstrates the potential ability to develop an optimized technique for recovery of this pathogen from water.

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Submitted date: 20 June 2003
Published date: 2004


Local EPrints ID: 30164
ISSN: 0099-2240
PURE UUID: 752e6847-00f6-4abc-ac41-251e7dc8564d
ORCID for C.W. Keevil: ORCID iD

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Date deposited: 11 May 2006
Last modified: 17 Jul 2017 15:55

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Author: N.F. Azevedo
Author: A.P. Pacheco
Author: C.W. Keevil ORCID iD
Author: M.J. Vieira

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