The University of Southampton
University of Southampton Institutional Repository

Biosensors for unattended cost-effective and continuous monitoring of environmental pollution: Automated water analyser computer supported system (AWACSS) and river analyser (RIANA)

Biosensors for unattended cost-effective and continuous monitoring of environmental pollution: Automated water analyser computer supported system (AWACSS) and river analyser (RIANA)
Biosensors for unattended cost-effective and continuous monitoring of environmental pollution: Automated water analyser computer supported system (AWACSS) and river analyser (RIANA)
This work describes our recent progress and achievements in the field of fully automated biosensors (Automated Water Analyser Computer Supported System (AWACSS) and River Analyser (RIANA)) for unattended, cost-effective and continuous monitoring of environmental pollution. We report on ultra-sensitive immunoassays for the hormones progesterone, testosterone and estrone and the pesticides propanil and isoproturon as examples of the outstanding progress made on biosensors in the field of environmental monitoring and water analysis. Most of the bio-active organic pollutants (estrone, progesterone, propanil and isoproturon) were detected at levels as low as 1.0pgm/L or even below.

In fact, the reported limits of detection (LOD) were between 0.2 and 6.0pgm/L. For the first time, commercially available derivatives and antibodies were incorporated into immunoassays (progesterone and testosterone) for fully automated biosensors. To verify the assay performance for quantifying testosterone, progesterone, and isoproturon in real-world samples using our immunosensors, we spiked river and drinking water at six different levels from 0.9pgm/L to 90ngm/L. Nearly all recovery rates could be obtained between 70 and 120% as the AOAC International recommends it chiefly for water analysis.
automated water analyser computer supported system, river analyser (riana), optical immunosensor, environmental analysis, bio-active organic pollutants, continuous monitoring
0306-7319
837-852
Tschmelak, Jens
aa5c4cb8-42fd-496e-a0ad-bbce99e2b568
Proll, Guenther
f0d76e96-e6e3-43af-8242-2ac92acf4d66
Riedt, Johannes
6a4f9744-83ff-4970-b323-b4f09ade39c4
Kaiser, Joachim
0e487776-21c4-44c9-a34d-bfa3cc6bd1cd
Kraemmer, Peter
aedb03c3-1807-4e28-92d6-42e2cb386984
Bárzaga, Luis
96470766-d13c-4d35-811d-ab29c64e012f
Wilkinson, James
73483cf3-d9f2-4688-9b09-1c84257884ca
Hua, Ping
92fa76e2-970b-45f5-a459-d9f95e735303
Hole, J. Patrick
0ce2d748-7b31-4ada-bad0-e9af21cdb192
Nudd, Richard
a52c6c1b-2ba7-44c3-b330-8d8e3e0e207e
Jackson, Michael
6ebc220f-eda6-497d-b761-0d053ccfd220
Abuknesha, Ram
dad4314b-6eb6-478e-8d9a-00cfc50bd0a1
Barceló, Damià
db19f2c1-8595-494f-ba36-1eb7e8994e64
Rodriguez-Mozaz, Sara
d9b0484d-34b7-466c-a476-7d3402ffd42d
López de Alda, Maria J.
e6392ffc-9a93-4570-a4e8-16d83f54aa7c
Sacher, Frank
4f7b7bed-c926-4cc0-b4b4-3af5a3da268a
Stien, Jan
3301ad81-ec56-45e0-ab7f-170db19f66a8
Slobodník, Jaroslav
3194ea75-fa81-4f70-a51a-ab59c4f721e8
Oswald, Peter
05c6e5dd-7449-4a79-87d9-1095df0b8b61
Kozmenko, Helena
ae84392f-231a-49ed-bb56-81bd348a3703
Korenková, Eva
5576893f-1f97-4040-837c-5e0f434948c6
Tóthová, Lívia
82e8ccf4-f9ae-4e8d-903c-a022d7f26ddd
Krascsenits, Zoltan
c395459e-3f7b-436c-873f-b23dd5f8b60b
Gauglitz, Guenter
c7a244e5-e7dc-4dce-a9fd-c83945597956
Tschmelak, Jens
aa5c4cb8-42fd-496e-a0ad-bbce99e2b568
Proll, Guenther
f0d76e96-e6e3-43af-8242-2ac92acf4d66
Riedt, Johannes
6a4f9744-83ff-4970-b323-b4f09ade39c4
Kaiser, Joachim
0e487776-21c4-44c9-a34d-bfa3cc6bd1cd
Kraemmer, Peter
aedb03c3-1807-4e28-92d6-42e2cb386984
Bárzaga, Luis
96470766-d13c-4d35-811d-ab29c64e012f
Wilkinson, James
73483cf3-d9f2-4688-9b09-1c84257884ca
Hua, Ping
92fa76e2-970b-45f5-a459-d9f95e735303
Hole, J. Patrick
0ce2d748-7b31-4ada-bad0-e9af21cdb192
Nudd, Richard
a52c6c1b-2ba7-44c3-b330-8d8e3e0e207e
Jackson, Michael
6ebc220f-eda6-497d-b761-0d053ccfd220
Abuknesha, Ram
dad4314b-6eb6-478e-8d9a-00cfc50bd0a1
Barceló, Damià
db19f2c1-8595-494f-ba36-1eb7e8994e64
Rodriguez-Mozaz, Sara
d9b0484d-34b7-466c-a476-7d3402ffd42d
López de Alda, Maria J.
e6392ffc-9a93-4570-a4e8-16d83f54aa7c
Sacher, Frank
4f7b7bed-c926-4cc0-b4b4-3af5a3da268a
Stien, Jan
3301ad81-ec56-45e0-ab7f-170db19f66a8
Slobodník, Jaroslav
3194ea75-fa81-4f70-a51a-ab59c4f721e8
Oswald, Peter
05c6e5dd-7449-4a79-87d9-1095df0b8b61
Kozmenko, Helena
ae84392f-231a-49ed-bb56-81bd348a3703
Korenková, Eva
5576893f-1f97-4040-837c-5e0f434948c6
Tóthová, Lívia
82e8ccf4-f9ae-4e8d-903c-a022d7f26ddd
Krascsenits, Zoltan
c395459e-3f7b-436c-873f-b23dd5f8b60b
Gauglitz, Guenter
c7a244e5-e7dc-4dce-a9fd-c83945597956

Tschmelak, Jens, Proll, Guenther, Riedt, Johannes, Kaiser, Joachim, Kraemmer, Peter, Bárzaga, Luis, Wilkinson, James, Hua, Ping, Hole, J. Patrick, Nudd, Richard, Jackson, Michael, Abuknesha, Ram, Barceló, Damià, Rodriguez-Mozaz, Sara, López de Alda, Maria J., Sacher, Frank, Stien, Jan, Slobodník, Jaroslav, Oswald, Peter, Kozmenko, Helena, Korenková, Eva, Tóthová, Lívia, Krascsenits, Zoltan and Gauglitz, Guenter (2005) Biosensors for unattended cost-effective and continuous monitoring of environmental pollution: Automated water analyser computer supported system (AWACSS) and river analyser (RIANA). International Journal of Environmental Analytical Chemistry, 85 (12-13), 837-852. (doi:10.1080/03067310500149619).

Record type: Article

Abstract

This work describes our recent progress and achievements in the field of fully automated biosensors (Automated Water Analyser Computer Supported System (AWACSS) and River Analyser (RIANA)) for unattended, cost-effective and continuous monitoring of environmental pollution. We report on ultra-sensitive immunoassays for the hormones progesterone, testosterone and estrone and the pesticides propanil and isoproturon as examples of the outstanding progress made on biosensors in the field of environmental monitoring and water analysis. Most of the bio-active organic pollutants (estrone, progesterone, propanil and isoproturon) were detected at levels as low as 1.0pgm/L or even below.

In fact, the reported limits of detection (LOD) were between 0.2 and 6.0pgm/L. For the first time, commercially available derivatives and antibodies were incorporated into immunoassays (progesterone and testosterone) for fully automated biosensors. To verify the assay performance for quantifying testosterone, progesterone, and isoproturon in real-world samples using our immunosensors, we spiked river and drinking water at six different levels from 0.9pgm/L to 90ngm/L. Nearly all recovery rates could be obtained between 70 and 120% as the AOAC International recommends it chiefly for water analysis.

Full text not available from this repository.

More information

Published date: 2005
Keywords: automated water analyser computer supported system, river analyser (riana), optical immunosensor, environmental analysis, bio-active organic pollutants, continuous monitoring

Identifiers

Local EPrints ID: 30197
URI: https://eprints.soton.ac.uk/id/eprint/30197
ISSN: 0306-7319
PURE UUID: a1b0a1ff-8170-4b54-a883-9ffe379035b3
ORCID for James Wilkinson: ORCID iD orcid.org/0000-0003-4712-1697

Catalogue record

Date deposited: 11 May 2006
Last modified: 18 Jul 2019 01:26

Export record

Altmetrics

Contributors

Author: Jens Tschmelak
Author: Guenther Proll
Author: Johannes Riedt
Author: Joachim Kaiser
Author: Peter Kraemmer
Author: Luis Bárzaga
Author: James Wilkinson ORCID iD
Author: Ping Hua
Author: J. Patrick Hole
Author: Richard Nudd
Author: Michael Jackson
Author: Ram Abuknesha
Author: Damià Barceló
Author: Sara Rodriguez-Mozaz
Author: Maria J. López de Alda
Author: Frank Sacher
Author: Jan Stien
Author: Jaroslav Slobodník
Author: Peter Oswald
Author: Helena Kozmenko
Author: Eva Korenková
Author: Lívia Tóthová
Author: Zoltan Krascsenits
Author: Guenter Gauglitz

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of https://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×