The University of Southampton
University of Southampton Institutional Repository

Regulation by GD3 of the proinflammatory response of microglia mediated by interleukin-15

Record type: Article

The interleukin (IL)-15-dependent immune responses of murine microglia were strongly affected by low concentrations of the ganglioside GD3. The ganglioside binding to IL-15 inhibited the proinflammatory effects of the cytokine, reducing IL-15-dependent T-cell proliferation as well as mRNA expression for IL-15Ralpha, p65, and NFATc2 in the N13 murine microglial cell line. Treatment of primary murine microglial cultures with GD3 abolished IL-15 production, without affecting cellular viability, but decreased the production of nitric oxide, a direct sensor of inflammation and nuclear factor-kappaB activity. We conclude that low doses of GD3 could inhibit specific proinflammatory mechanisms and modulate the inflammatory environment, leading to a less reactive scene. Microglial cells are one of the main actors in the inflammatory events that follow CNS trauma or an autoimmune disease episode, modulating the internal production of cytokines, growth factors, and other homeostatic molecules that may determine the evolution and outcome of tissue damage. Proinflammatory cytokines have a relevant role in the initial events, and modulation of their activity by gangliosides could cut down their harmful effects and interfere with invasion of the CNS by peripheral immune cells. The antiinflammatory properties of GD3 could be significant in the treatment of pain subsequent to CNS damage.

Full text not available from this repository.

Citation

Gomez-Nicola, Diego, Doncel-Pérez, Ernesto and Nieto-Sampedro, Manuel (2006) Regulation by GD3 of the proinflammatory response of microglia mediated by interleukin-15 Journal of Neuroscience Research, 83, (5), pp. 754-762. (doi:10.1002/jnr.20777). (PMID:16477650).

More information

Published date: April 2006
Keywords: CNS, neuropathic pain, cytokines, gangliosides
Organisations: Biomedicine

Identifiers

Local EPrints ID: 333266
URI: http://eprints.soton.ac.uk/id/eprint/333266
ISSN: 0360-4012
PURE UUID: 08453318-4bfc-4645-9a71-dfb8ab90a3a5
ORCID for Diego Gomez-Nicola: ORCID iD orcid.org/0000-0002-5316-2682

Catalogue record

Date deposited: 14 Mar 2012 13:40
Last modified: 18 Jul 2017 06:13

Export record

Altmetrics

Contributors

Author: Ernesto Doncel-Pérez
Author: Manuel Nieto-Sampedro

University divisions


Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×