The University of Southampton
University of Southampton Institutional Repository

Site specific increase in heterogeneity of trabecular bone tissue mineral during oestrogen deficiency

Site specific increase in heterogeneity of trabecular bone tissue mineral during oestrogen deficiency
Site specific increase in heterogeneity of trabecular bone tissue mineral during oestrogen deficiency
Although osteoporosis reduces overall bone mass causing bone fragility, recent studies report that the remaining bone tissue is significantly stiffer. Preliminary studies indicate that alterations in bone tissue mineral content might explain these changes, albeit that other studies report conflicting observations. The objective of this study is to quantify whether the distribution of bone tissue mineral is altered during oestrogen deficiency. Individual trabeculae were harvested from the proximal femur of 7 ovariectomised sheep (OVX), sacrificed 12 months post-surgery, and 5 age-matched controls. Mineral content (wt% Ca) was determined using a quantitative backscattered scanning electron microscopy imaging approach. Mineral heterogeneity within individual trabeculae was compared by calculating the full width at half maximum (FWHM) of mineral density distributions. Mean calcium content, the spatial distribution of mineral within trabeculae and the inter-trabecular variation between regions of proximal femora were also compared. Oestrogen deficiency increased mineral heterogeneity within individual trabeculae compared to healthy controls, as measured by FWHM (3.57 ± 0.68 vs. 3.17 ± 0.36 wt% Ca, p = 0.04). In particular mineral variability increased between superficial and deep regions of trabeculae of OVX animals (p = 0.04). Interestingly, mineralisation variability between greater and lesser trochanters (i.e. intertrochanteric fracture line) was increased in OVX compared to CON, as indicated by a greater % difference in the standard deviation of trabecular mineral content (77.11 ± 11.70 vs. 45.64 ± 23.70 %, p = 0.03). Such changes are undetectable by evaluating the mean mineral content of bone tissue, but may contribute to changes in bone mechanical strength following osteoporotic bone loss.
396-406
Brennan, M.A.
3bdafd88-03b9-4856-b97b-e0bec5f46304
Gleeson, J. P.
42225cec-983b-4f17-b13f-16e59715e2cd
Browne, M.
6578cc37-7bd6-43b9-ae5c-77ccb7726397
O'Brien, F.J.
e3fa4bfe-8bda-459c-aa1a-e8048c66d86d
Thurner, P. J.
ab711ddd-784e-48de-aaad-f56aec40f84f
McNamara, L.M.
328e6cb9-8295-43d5-86d5-f9fed780f491
Brennan, M.A.
3bdafd88-03b9-4856-b97b-e0bec5f46304
Gleeson, J. P.
42225cec-983b-4f17-b13f-16e59715e2cd
Browne, M.
6578cc37-7bd6-43b9-ae5c-77ccb7726397
O'Brien, F.J.
e3fa4bfe-8bda-459c-aa1a-e8048c66d86d
Thurner, P. J.
ab711ddd-784e-48de-aaad-f56aec40f84f
McNamara, L.M.
328e6cb9-8295-43d5-86d5-f9fed780f491

Brennan, M.A., Gleeson, J. P., Browne, M., O'Brien, F.J., Thurner, P. J. and McNamara, L.M. (2011) Site specific increase in heterogeneity of trabecular bone tissue mineral during oestrogen deficiency. European Cells & Materials, 21, 396-406. (PMID:21574136)

Record type: Article

Abstract

Although osteoporosis reduces overall bone mass causing bone fragility, recent studies report that the remaining bone tissue is significantly stiffer. Preliminary studies indicate that alterations in bone tissue mineral content might explain these changes, albeit that other studies report conflicting observations. The objective of this study is to quantify whether the distribution of bone tissue mineral is altered during oestrogen deficiency. Individual trabeculae were harvested from the proximal femur of 7 ovariectomised sheep (OVX), sacrificed 12 months post-surgery, and 5 age-matched controls. Mineral content (wt% Ca) was determined using a quantitative backscattered scanning electron microscopy imaging approach. Mineral heterogeneity within individual trabeculae was compared by calculating the full width at half maximum (FWHM) of mineral density distributions. Mean calcium content, the spatial distribution of mineral within trabeculae and the inter-trabecular variation between regions of proximal femora were also compared. Oestrogen deficiency increased mineral heterogeneity within individual trabeculae compared to healthy controls, as measured by FWHM (3.57 ± 0.68 vs. 3.17 ± 0.36 wt% Ca, p = 0.04). In particular mineral variability increased between superficial and deep regions of trabeculae of OVX animals (p = 0.04). Interestingly, mineralisation variability between greater and lesser trochanters (i.e. intertrochanteric fracture line) was increased in OVX compared to CON, as indicated by a greater % difference in the standard deviation of trabecular mineral content (77.11 ± 11.70 vs. 45.64 ± 23.70 %, p = 0.03). Such changes are undetectable by evaluating the mean mineral content of bone tissue, but may contribute to changes in bone mechanical strength following osteoporotic bone loss.

Text
v021a30.pdf - Other
Download (284kB)

More information

Published date: 2011
Organisations: Bioengineering Group

Identifiers

Local EPrints ID: 333588
URI: https://eprints.soton.ac.uk/id/eprint/333588
PURE UUID: b99bfe26-40ac-4170-b16b-e2f602ab47f6
ORCID for M. Browne: ORCID iD orcid.org/0000-0001-5184-050X

Catalogue record

Date deposited: 07 Mar 2012 14:08
Last modified: 06 Jun 2018 13:03

Export record

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of https://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×