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Contrast agent-free cell sonoporation using a continuous-flow microfluidic device

Contrast agent-free cell sonoporation using a continuous-flow microfluidic device
Contrast agent-free cell sonoporation using a continuous-flow microfluidic device
Ultrasound (US) has been utilised to induce reversible poration of cell membranes (sonoporation) in order to facilitate the trans-membrane delivery of pharmaceutical agents. Sonoporation is conventionally performed in the presence of microbubble contrast agents (CA). However, challenges remain in achieving controlled sonoporation due to CA cavitational effects, which can significantly reduce cell viability. Therefore CA-free sonoporation represents a “gentle” methodology for facilitating the delivery of pharmaceutical agents while maintaining high cell viability. We report facilitated uptake of doxorubicin and apigenin by CA free sonoporation of suspended cardiomyocytes by generating an ultrasonic standing wave within an easy to-use continuous-flow microfluidic device
Carugo, Dario
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Ankrett, Dyan N.
22d8eb6d-70db-4b47-9587-c4d048cd34b7
Glynne-Jones, Peter
6ca3fcbc-14db-4af9-83e2-cf7c8b91ef0d
Capretto, Lorenzo
0f3586b5-1560-49c1-a76b-59e74ea600ef
Boltryk, Rosemary J.
0452b21c-a758-4d4a-925b-1511d9296d62
Townsend, Paul A.
a2680443-664e-46d0-b4dd-97456ba810db
Zhang, Xunli
d7cf1181-3276-4da1-9150-e212b333abb1
Hill, Martyn
0cda65c8-a70f-476f-b126-d2c4460a253e
Carugo, Dario
0a4be6cd-e309-4ed8-a620-20256ce01179
Ankrett, Dyan N.
22d8eb6d-70db-4b47-9587-c4d048cd34b7
Glynne-Jones, Peter
6ca3fcbc-14db-4af9-83e2-cf7c8b91ef0d
Capretto, Lorenzo
0f3586b5-1560-49c1-a76b-59e74ea600ef
Boltryk, Rosemary J.
0452b21c-a758-4d4a-925b-1511d9296d62
Townsend, Paul A.
a2680443-664e-46d0-b4dd-97456ba810db
Zhang, Xunli
d7cf1181-3276-4da1-9150-e212b333abb1
Hill, Martyn
0cda65c8-a70f-476f-b126-d2c4460a253e

Carugo, Dario, Ankrett, Dyan N., Glynne-Jones, Peter, Capretto, Lorenzo, Boltryk, Rosemary J., Townsend, Paul A., Zhang, Xunli and Hill, Martyn (2011) Contrast agent-free cell sonoporation using a continuous-flow microfluidic device. 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Seattle, United States. 02 - 06 Oct 2011.

Record type: Conference or Workshop Item (Paper)

Abstract

Ultrasound (US) has been utilised to induce reversible poration of cell membranes (sonoporation) in order to facilitate the trans-membrane delivery of pharmaceutical agents. Sonoporation is conventionally performed in the presence of microbubble contrast agents (CA). However, challenges remain in achieving controlled sonoporation due to CA cavitational effects, which can significantly reduce cell viability. Therefore CA-free sonoporation represents a “gentle” methodology for facilitating the delivery of pharmaceutical agents while maintaining high cell viability. We report facilitated uptake of doxorubicin and apigenin by CA free sonoporation of suspended cardiomyocytes by generating an ultrasonic standing wave within an easy to-use continuous-flow microfluidic device

Full text not available from this repository.

More information

Published date: October 2011
Venue - Dates: 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences, Seattle, United States, 2011-10-02 - 2011-10-06
Organisations: Cancer Sciences, Bioengineering Group, Mechatronics

Identifiers

Local EPrints ID: 333944
URI: https://eprints.soton.ac.uk/id/eprint/333944
PURE UUID: a21b892f-a7c8-4cf2-996d-053b0a286542
ORCID for Peter Glynne-Jones: ORCID iD orcid.org/0000-0001-5684-3953
ORCID for Martyn Hill: ORCID iD orcid.org/0000-0001-6448-9448

Catalogue record

Date deposited: 08 Mar 2012 11:44
Last modified: 14 Dec 2018 01:38

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