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Up-regulation of p21WAF1/CIP1 in psoriasis and after the application of irritants and tape stripping

Record type: Article

p21WAF1/CIP1 is a nucleoprotein that was initially characterized by its ability to be regulated transcriptionally by p53 and by its ability to mediate growth arrest by binding to cyclin-dependent kinases. Although p21WAF1/CIP1 is thought to mediate the effects of p53 in causing growth arrest, p21WAF1/CIP1 is also regulated in a p53-independent manner, e.g., during terminal differentiation of some cell lines. Growth factors including epidermal growth factor also induce p21WAF1/CIP1 through p53-independent pathways. Because the epidermal growth factor signaling pathway is abnormal in psoriatic epidermis, we studied p21WAF1/CIP1 expression, using in situ hybridization and immunohistochemistry, in psoriasis. Both p21WAF1/CIP1 mRNA and protein were significantly elevated in untreated psoriatic plaques compared with uninvolved psoriatic skin (p < 0.0001), with the up-regulation of p21WAF1/CIP1 being predominantly suprabasal. This increase was accompanied by a small increase in p53 protein expression of uncertain significance. Furthermore, p21WAF1/CIP1 expression was induced in skin after sellotape stripping and by the application of agents, such as dithranol, that are capable of inducing hyperproliferation. The pattern of p21WAF1/CIP1 expression observed is consistent with a role in induction and maintenance of differentiation. Our experiments, however, cannot determine whether the abnormalities of p21WAF1/CIP1 epidermal expression in psoriasis and after insult are independent of changes in p53 expression.

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Citation

Healy, Eugene, Reynolds, Nicholas J., Smith, Martin D., Harrison, David, Doherty, Elaine, Campbell, Christine and Rees, Jonathan L. (1995) Up-regulation of p21WAF1/CIP1 in psoriasis and after the application of irritants and tape stripping Journal of Investigative Dermatology, 105, (2), pp. 274-279. (doi:10.1111/1523-1747.ep12318430). (PMID:7636313).

More information

Published date: August 1995
Keywords: differentiation, dithranol, p53, proliferation
Organisations: Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 334170
URI: http://eprints.soton.ac.uk/id/eprint/334170
ISSN: 0022-202X
PURE UUID: e0be4252-b0c4-4b5d-8e42-5ca263f81857

Catalogue record

Date deposited: 14 Mar 2012 14:01
Last modified: 18 Jul 2017 06:12

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Contributors

Author: Eugene Healy
Author: Nicholas J. Reynolds
Author: Martin D. Smith
Author: David Harrison
Author: Elaine Doherty
Author: Christine Campbell
Author: Jonathan L. Rees

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