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Thin film polycrystalline silicon nanowire biosensors

Thin film polycrystalline silicon nanowire biosensors
Thin film polycrystalline silicon nanowire biosensors
Polysilicon nanowire biosensors have been fabricated using a top-down process and were used to determine the binding constant of two inflammatory biomarkers. A very low cost nanofabrication process was developed, based on simple and mature photolithography, thin film technology and plasma etching, enabling an easy route to mass manufacture. Antibodyfunctionalized nanowire sensors were used to detect the proteins interleukin-8 (IL-8) and tumor necrosis factor-alpha (TNF-alpha) over a wide range of concentrations, demonstrating excellent sensitivity and selectivity, exemplified by a detection sensitivity of 10fM in the presence of a 100,000-fold excess of a non-target protein. Nanowire titration curves gave antibody-antigen dissociation constants in good agreement with low-salt enzyme-linked immunosorbent assays (ELISAs). This fabrication process produces high-quality nanowires that are suitable for lowcost mass production, providing a realistic route to the realization of disposable nanoelectronic Point-of-Care (PoC) devices.
1530-6984
1868-1872
Al Hakim, Mohammad M.
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Lombardini, Marta
c8653079-b66c-49ca-ac59-93c3d17b26a4
Sun, Kai
2ace53c1-82cc-47e5-911e-898b143163d3
Giustiniano, Francesco
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Roach, Peter L.
ca94060c-4443-482b-af3e-979243488ba9
Davies, Donna E.
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Howarth, Peter H.
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de Planque, Maurits R.R.
a1d33d13-f516-44fb-8d2c-c51d18bc21ba
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Ashburn, Peter
68cef6b7-205b-47aa-9efb-f1f09f5c1038
Al Hakim, Mohammad M.
b533c5cd-5683-48d8-a377-c8492a050cdb
Lombardini, Marta
c8653079-b66c-49ca-ac59-93c3d17b26a4
Sun, Kai
2ace53c1-82cc-47e5-911e-898b143163d3
Giustiniano, Francesco
f2692f6a-8f0a-49de-aa5d-7c2dff402545
Roach, Peter L.
ca94060c-4443-482b-af3e-979243488ba9
Davies, Donna E.
7de8fdc7-3640-4e3a-aa91-d0e03f990c38
Howarth, Peter H.
ff19c8c4-86b0-4a88-8f76-b3d87f142a21
de Planque, Maurits R.R.
a1d33d13-f516-44fb-8d2c-c51d18bc21ba
Morgan, Hywel
de00d59f-a5a2-48c4-a99a-1d5dd7854174
Ashburn, Peter
68cef6b7-205b-47aa-9efb-f1f09f5c1038

Al Hakim, Mohammad M., Lombardini, Marta, Sun, Kai, Giustiniano, Francesco, Roach, Peter L., Davies, Donna E., Howarth, Peter H., de Planque, Maurits R.R., Morgan, Hywel and Ashburn, Peter (2012) Thin film polycrystalline silicon nanowire biosensors. Nano Letters, 12 (4), 1868-1872. (doi:10.1021/nl2042276). (PMID:22432636)

Record type: Article

Abstract

Polysilicon nanowire biosensors have been fabricated using a top-down process and were used to determine the binding constant of two inflammatory biomarkers. A very low cost nanofabrication process was developed, based on simple and mature photolithography, thin film technology and plasma etching, enabling an easy route to mass manufacture. Antibodyfunctionalized nanowire sensors were used to detect the proteins interleukin-8 (IL-8) and tumor necrosis factor-alpha (TNF-alpha) over a wide range of concentrations, demonstrating excellent sensitivity and selectivity, exemplified by a detection sensitivity of 10fM in the presence of a 100,000-fold excess of a non-target protein. Nanowire titration curves gave antibody-antigen dissociation constants in good agreement with low-salt enzyme-linked immunosorbent assays (ELISAs). This fabrication process produces high-quality nanowires that are suitable for lowcost mass production, providing a realistic route to the realization of disposable nanoelectronic Point-of-Care (PoC) devices.

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Published date: 20 March 2012
Organisations: Nanoelectronics and Nanotechnology, Organic Chemistry: SCF, Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 336302
URI: https://eprints.soton.ac.uk/id/eprint/336302
ISSN: 1530-6984
PURE UUID: 8c4d3bef-4669-4224-a6ff-6e317353e5f2
ORCID for Francesco Giustiniano: ORCID iD orcid.org/0000-0001-9277-8538
ORCID for Peter L. Roach: ORCID iD orcid.org/0000-0001-9880-2877
ORCID for Donna E. Davies: ORCID iD orcid.org/0000-0002-5117-2991
ORCID for Maurits R.R. de Planque: ORCID iD orcid.org/0000-0002-8787-0513
ORCID for Hywel Morgan: ORCID iD orcid.org/0000-0003-4850-5676

Catalogue record

Date deposited: 21 Mar 2012 13:33
Last modified: 19 Jul 2019 01:24

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Contributors

Author: Mohammad M. Al Hakim
Author: Marta Lombardini
Author: Kai Sun
Author: Peter L. Roach ORCID iD
Author: Donna E. Davies ORCID iD
Author: Hywel Morgan ORCID iD
Author: Peter Ashburn

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