The University of Southampton
University of Southampton Institutional Repository

Evaluation of methylation status of the eNOS promoter at birth in relation to childhood bone mineral content

Evaluation of methylation status of the eNOS promoter at birth in relation to childhood bone mineral content
Evaluation of methylation status of the eNOS promoter at birth in relation to childhood bone mineral content
Our previous work has shown associations between childhood adiposity and perinatal methylation status of several genes in umbilical cord tissue, including endothelial nitric oxide synthase (eNOS). There is increasing evidence that eNOS is important in bone metabolism; we therefore related the methylation status of the eNOS gene promoter in stored umbilical cord to childhood bone size and density in a group of 9-year-old children. We used Sequenom MassARRAY to assess the methylation status of two CpGs in the eNOS promoter, identified from our previous study, in stored umbilical cords of 66 children who formed part of a Southampton birth cohort and who had measurements of bone size and density at age 9 years (Lunar DPXL DXA instrument). Percentage methylation varied greatly between subjects. For one of the two CpGs, eNOS chr7:150315553 + , after taking account of age and sex, there were strong positive associations between methylation status and the child’s whole-body bone area (r = 0.28, P = 0.02), bone mineral content (r = 0.34, P = 0.005), and areal bone mineral density (r = 0.34, P = 0.005) at age 9 years. These associations were independent of previously documented maternal determinants of offspring bone mass. Our findings suggest an association between methylation status at birth of a specific CpG within the eNOS promoter and bone mineral content in childhood. This supports a role for eNOS in bone growth and metabolism and implies that its contribution may at least in part occur during early skeletal development.
epigenesis, methylation, umbilical cord, eNOS, DXA
0171-967X
120-127
Harvey, Nicholas C.
ce487fb4-d360-4aac-9d17-9466d6cba145
Lillycrop, Karen A.
eeaaa78d-0c4d-4033-a178-60ce7345a2cc
Garratt, Emma
863257ac-3150-4e33-b0ae-1d9d4e8c6614
Sheppard, Allan
a6e27895-05ad-4b0b-98bb-4aa033b7182d
McLean, Cameron
245194c5-ff48-4da4-9e5f-c437dc67dae4
Burdge, Graham C.
09d60a07-8ca1-4351-9bf1-de6ffcfb2159
Slater-Jefferies, Jo
e46c711a-9d4c-436a-b853-828df69bb4d7
Rodford, Joanne
f3197216-697f-45fd-a090-8eb5e6c91bac
Crozier, Sarah
a9c88016-8f46-4659-954e-4d7af8a49594
Inskip, Hazel
5fb4470a-9379-49b2-a533-9da8e61058b7
Emerald, B.S.
36ae23a7-8650-4f3e-8af0-38413d49fdb3
Gale, C.R.
5bb2abb3-7b53-42d6-8aa7-817e193140c8
Hanson, M.
1952fad1-abc7-4284-a0bc-a7eb31f70a3f
Gluckman, P.
dadc86d4-4eaa-4589-b560-413a9e564558
Godfrey, K.
0931701e-fe2c-44b5-8f0d-ec5c7477a6fd
Cooper, C.
e05f5612-b493-4273-9b71-9e0ce32bdad6
Harvey, Nicholas C.
ce487fb4-d360-4aac-9d17-9466d6cba145
Lillycrop, Karen A.
eeaaa78d-0c4d-4033-a178-60ce7345a2cc
Garratt, Emma
863257ac-3150-4e33-b0ae-1d9d4e8c6614
Sheppard, Allan
a6e27895-05ad-4b0b-98bb-4aa033b7182d
McLean, Cameron
245194c5-ff48-4da4-9e5f-c437dc67dae4
Burdge, Graham C.
09d60a07-8ca1-4351-9bf1-de6ffcfb2159
Slater-Jefferies, Jo
e46c711a-9d4c-436a-b853-828df69bb4d7
Rodford, Joanne
f3197216-697f-45fd-a090-8eb5e6c91bac
Crozier, Sarah
a9c88016-8f46-4659-954e-4d7af8a49594
Inskip, Hazel
5fb4470a-9379-49b2-a533-9da8e61058b7
Emerald, B.S.
36ae23a7-8650-4f3e-8af0-38413d49fdb3
Gale, C.R.
5bb2abb3-7b53-42d6-8aa7-817e193140c8
Hanson, M.
1952fad1-abc7-4284-a0bc-a7eb31f70a3f
Gluckman, P.
dadc86d4-4eaa-4589-b560-413a9e564558
Godfrey, K.
0931701e-fe2c-44b5-8f0d-ec5c7477a6fd
Cooper, C.
e05f5612-b493-4273-9b71-9e0ce32bdad6

Harvey, Nicholas C., Lillycrop, Karen A., Garratt, Emma, Sheppard, Allan, McLean, Cameron, Burdge, Graham C., Slater-Jefferies, Jo, Rodford, Joanne, Crozier, Sarah, Inskip, Hazel, Emerald, B.S., Gale, C.R., Hanson, M., Gluckman, P., Godfrey, K. and Cooper, C. (2012) Evaluation of methylation status of the eNOS promoter at birth in relation to childhood bone mineral content. Calcified Tissue International, 90 (2), 120-127. (doi:10.1007/s00223-011-9554-5). (PMID:22159788)

Record type: Article

Abstract

Our previous work has shown associations between childhood adiposity and perinatal methylation status of several genes in umbilical cord tissue, including endothelial nitric oxide synthase (eNOS). There is increasing evidence that eNOS is important in bone metabolism; we therefore related the methylation status of the eNOS gene promoter in stored umbilical cord to childhood bone size and density in a group of 9-year-old children. We used Sequenom MassARRAY to assess the methylation status of two CpGs in the eNOS promoter, identified from our previous study, in stored umbilical cords of 66 children who formed part of a Southampton birth cohort and who had measurements of bone size and density at age 9 years (Lunar DPXL DXA instrument). Percentage methylation varied greatly between subjects. For one of the two CpGs, eNOS chr7:150315553 + , after taking account of age and sex, there were strong positive associations between methylation status and the child’s whole-body bone area (r = 0.28, P = 0.02), bone mineral content (r = 0.34, P = 0.005), and areal bone mineral density (r = 0.34, P = 0.005) at age 9 years. These associations were independent of previously documented maternal determinants of offspring bone mass. Our findings suggest an association between methylation status at birth of a specific CpG within the eNOS promoter and bone mineral content in childhood. This supports a role for eNOS in bone growth and metabolism and implies that its contribution may at least in part occur during early skeletal development.

Full text not available from this repository.

More information

e-pub ahead of print date: 8 December 2011
Published date: February 2012
Keywords: epigenesis, methylation, umbilical cord, eNOS, DXA
Organisations: Faculty of Medicine, Human Development & Health, Centre for Biological Sciences

Identifiers

Local EPrints ID: 336532
URI: http://eprints.soton.ac.uk/id/eprint/336532
ISSN: 0171-967X
PURE UUID: 5d4d33c8-8f00-4bb6-bb54-ffd370c86739
ORCID for Nicholas C. Harvey: ORCID iD orcid.org/0000-0002-8194-2512
ORCID for Karen A. Lillycrop: ORCID iD orcid.org/0000-0001-7350-5489
ORCID for Graham C. Burdge: ORCID iD orcid.org/0000-0002-7665-2967
ORCID for Jo Slater-Jefferies: ORCID iD orcid.org/0000-0001-8325-1320
ORCID for Hazel Inskip: ORCID iD orcid.org/0000-0001-8897-1749
ORCID for C.R. Gale: ORCID iD orcid.org/0000-0002-3361-8638
ORCID for M. Hanson: ORCID iD orcid.org/0000-0002-6907-613X
ORCID for K. Godfrey: ORCID iD orcid.org/0000-0002-4643-0618
ORCID for C. Cooper: ORCID iD orcid.org/0000-0003-3510-0709

Catalogue record

Date deposited: 30 Mar 2012 15:09
Last modified: 18 Feb 2021 17:06

Export record

Altmetrics

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×