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The rhodanese domain of ThiI is both necessary and sufficient for synthesis of the thiazole moiety of thiamine in Salmonella enterica

The rhodanese domain of ThiI is both necessary and sufficient for synthesis of the thiazole moiety of thiamine in Salmonella enterica
The rhodanese domain of ThiI is both necessary and sufficient for synthesis of the thiazole moiety of thiamine in Salmonella enterica
In Salmonella enterica, ThiI is a bifunctional enzyme required for the synthesis of both the 4-thiouridine modification in tRNA and the thiazole moiety of thiamine. In thiouridine biosynthesis, ThiI adenylates the tRNA uridine and transfers sulfur from a persulfide formed on the protein. The role of ThiI in thiazole synthesis is not yet well understood. Mutational analysis described herein identified ThiI residues required for thiouridine synthesis were not involved in thiazole biosynthesis. The data further showed that the C-terminal rhodanese domain of ThiI was sufficient for thiazole synthesis in vivo. Together these data support the conclusion that sulfur mobilization in thiazole synthesis is mechanistically distinct from that in 4-thiouridine synthesis, and suggest functional annotation of ThiI in genome sequences should be readdressed. Nutritional studies described herein identified an additional cysteine-dependent mechanism for sulfur mobilization to thiazole that did not require ThiI, IscS, SufS or glutathione. The later mechanism may provide insights into the chemistry used for sulfur mobilization to thiazole in organisms that do not utilize ThiI.
thiazole synthesis, thiamine, ThiI, rhodanese domains, sulfur mobilization
0021-9193
4582-4587
Martinez-Gomez, N. Cecilia
8cb2bb80-b536-400a-9fdd-b25df9b3f072
Palmer, Lauren D.
20fb70f6-dd40-493b-a058-b985a3e929e9
Vivas, Eugenio
aa793e71-59da-45b6-aca5-f3c030883516
Roach, Peter L.
ca94060c-4443-482b-af3e-979243488ba9
Downs, Diana M.
6520d430-c25e-439e-a49b-a8f7be75304f
Martinez-Gomez, N. Cecilia
8cb2bb80-b536-400a-9fdd-b25df9b3f072
Palmer, Lauren D.
20fb70f6-dd40-493b-a058-b985a3e929e9
Vivas, Eugenio
aa793e71-59da-45b6-aca5-f3c030883516
Roach, Peter L.
ca94060c-4443-482b-af3e-979243488ba9
Downs, Diana M.
6520d430-c25e-439e-a49b-a8f7be75304f

Martinez-Gomez, N. Cecilia, Palmer, Lauren D., Vivas, Eugenio, Roach, Peter L. and Downs, Diana M. (2011) The rhodanese domain of ThiI is both necessary and sufficient for synthesis of the thiazole moiety of thiamine in Salmonella enterica. Journal of Bacteriology, 193 (18), 4582-4587. (doi:10.1128/Jb.05325-11). (PMID:21724998)

Record type: Article

Abstract

In Salmonella enterica, ThiI is a bifunctional enzyme required for the synthesis of both the 4-thiouridine modification in tRNA and the thiazole moiety of thiamine. In thiouridine biosynthesis, ThiI adenylates the tRNA uridine and transfers sulfur from a persulfide formed on the protein. The role of ThiI in thiazole synthesis is not yet well understood. Mutational analysis described herein identified ThiI residues required for thiouridine synthesis were not involved in thiazole biosynthesis. The data further showed that the C-terminal rhodanese domain of ThiI was sufficient for thiazole synthesis in vivo. Together these data support the conclusion that sulfur mobilization in thiazole synthesis is mechanistically distinct from that in 4-thiouridine synthesis, and suggest functional annotation of ThiI in genome sequences should be readdressed. Nutritional studies described herein identified an additional cysteine-dependent mechanism for sulfur mobilization to thiazole that did not require ThiI, IscS, SufS or glutathione. The later mechanism may provide insights into the chemistry used for sulfur mobilization to thiazole in organisms that do not utilize ThiI.

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More information

Published date: September 2011
Keywords: thiazole synthesis, thiamine, ThiI, rhodanese domains, sulfur mobilization
Organisations: Chemistry

Identifiers

Local EPrints ID: 336607
URI: http://eprints.soton.ac.uk/id/eprint/336607
ISSN: 0021-9193
PURE UUID: 08b8d98b-d11d-42a0-a773-de39e46a8d1e
ORCID for Peter L. Roach: ORCID iD orcid.org/0000-0001-9880-2877

Catalogue record

Date deposited: 30 Mar 2012 12:45
Last modified: 26 Nov 2019 01:52

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