Synthesis and evaluation of a new non-fluorescent quencher in fluorogenic oligonucleotide probes for real-time PCR
Synthesis and evaluation of a new non-fluorescent quencher in fluorogenic oligonucleotide probes for real-time PCR
A non-fluorescent quencher, based on the diaminoanthraquinone Disperse Blue 3, has been incorporated into oligonucleotides at the 5?-end, the 3?-end and internally as a thymidine derivative. Fluorimetry and fluorogenic real-time PCR experiments demonstrate that the quencher is effective with a wide range of fluorescent dyes. The anthraquinone moiety increases the melting temperature of DNA duplexes, thus allowing shorter, more discriminatory probes to be used. The quencher has been used in Scorpion primers and TaqMan ® probes for human DNA sequence recognition and mutation detection.
cystic-fibrosis gene, scorpion primers, deoxynucleoside
phosphoramidites, molecular beacons, dna, identification, hybridization, fluorescence, polymerase, sequence
2534-2542
May, JP
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Brown, LJ
75aa95fa-5d27-46a7-9dbe-0f465a664f5b
van Delft, I
0509bb2a-8b1a-43cc-b092-6c878d7ac91b
Thelwell, Nicky
c94f6f0c-3fd0-4d68-b32a-3e4e2f47afab
Harley, K
84ef368f-6263-45c9-909b-c8e06f6059fc
Brown, T
a64aae36-bb30-42df-88a2-11be394e8c89
2005
May, JP
b54a262b-9f32-4435-8866-3b9c495294f3
Brown, LJ
75aa95fa-5d27-46a7-9dbe-0f465a664f5b
van Delft, I
0509bb2a-8b1a-43cc-b092-6c878d7ac91b
Thelwell, Nicky
c94f6f0c-3fd0-4d68-b32a-3e4e2f47afab
Harley, K
84ef368f-6263-45c9-909b-c8e06f6059fc
Brown, T
a64aae36-bb30-42df-88a2-11be394e8c89
May, JP, Brown, LJ, van Delft, I, Thelwell, Nicky, Harley, K and Brown, T
(2005)
Synthesis and evaluation of a new non-fluorescent quencher in fluorogenic oligonucleotide probes for real-time PCR.
Organic & Biomolecular Chemistry, 3 (14), .
(doi:10.1039/B504759E).
Abstract
A non-fluorescent quencher, based on the diaminoanthraquinone Disperse Blue 3, has been incorporated into oligonucleotides at the 5?-end, the 3?-end and internally as a thymidine derivative. Fluorimetry and fluorogenic real-time PCR experiments demonstrate that the quencher is effective with a wide range of fluorescent dyes. The anthraquinone moiety increases the melting temperature of DNA duplexes, thus allowing shorter, more discriminatory probes to be used. The quencher has been used in Scorpion primers and TaqMan ® probes for human DNA sequence recognition and mutation detection.
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e-pub ahead of print date: 7 July 2005
Published date: 2005
Keywords:
cystic-fibrosis gene, scorpion primers, deoxynucleoside
phosphoramidites, molecular beacons, dna, identification, hybridization, fluorescence, polymerase, sequence
Organisations:
Chemistry
Identifiers
Local EPrints ID: 337199
URI: http://eprints.soton.ac.uk/id/eprint/337199
ISSN: 1477-0520
PURE UUID: e4b9030b-8032-44bc-b3e6-cfb0a6a3f358
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Date deposited: 20 Apr 2012 12:46
Last modified: 15 Mar 2024 04:03
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Author:
I van Delft
Author:
Nicky Thelwell
Author:
K Harley
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