The University of Southampton
University of Southampton Institutional Repository

Uniform aligned bioconjugation of biomolecule motifs for integration within microfabricated microfluidic devices

Uniform aligned bioconjugation of biomolecule motifs for integration within microfabricated microfluidic devices
Uniform aligned bioconjugation of biomolecule motifs for integration within microfabricated microfluidic devices
Full details and a step-by-step guide suitable for printing proteins aligned to micron-sized sensors and subsequent integration and alignment of microfluidic structures are presented. The precise alignment and grafting of micron-sized biomolecule patterns with an underlying substrate at predefined locations is achieved using a novel semi-automated microcontact printer. Through integration of optical alignment methods in the x, y, and z directions, uniform contact of micron-sized stamps is achieved. Feature compression of the stamp is avoided by fine control of the stamp during contact. This printing method has been developed in combination with robust, compatible bioconjugate chemistry for patterning of a dextran-functionalized silicon oxide substrate with a NeutrAvidin-"inked" stamp and subsequent incubation with a biotin-functionalized protein. The bioconjugate chemistry is such that uniform coverage of the protein (without denaturation) over the printed motif is obtained and reproduction of the initial mask shape and dimensions is achieved. Later integration with a microfluidic structure aligned with the printed motif on the substrate is also described.
microfluidic, biosensors, bioconjugation, bioaffinity sensors, lab-on-a-chip, microcontact printing, microfabrication
0003-2697
195-205
Braddick, Lucy M.
d74c6a4c-d20f-4ca4-ac4f-b1c1d4fa978a
Garland, Patrick J.
1d24a0cc-81f2-4ef1-82bd-77d2510e59d6
Praeger, Matthew F.
84575f28-4530-4f89-9355-9c5b6acc6cac
Butement, Jonathan
581ce321-f1af-4a2f-870a-9d8d45133586
Friedrich, Daniel
38fec5ed-5104-4d67-9354-2e064d0bdad2
Morgan, David J.
a54eaa5b-09bc-4185-8f18-dfc1eeeb310e
Melvin, Tracy
fd87f5eb-2bb9-48fa-b7be-7100ace9c50f
Braddick, Lucy M.
d74c6a4c-d20f-4ca4-ac4f-b1c1d4fa978a
Garland, Patrick J.
1d24a0cc-81f2-4ef1-82bd-77d2510e59d6
Praeger, Matthew F.
84575f28-4530-4f89-9355-9c5b6acc6cac
Butement, Jonathan
581ce321-f1af-4a2f-870a-9d8d45133586
Friedrich, Daniel
38fec5ed-5104-4d67-9354-2e064d0bdad2
Morgan, David J.
a54eaa5b-09bc-4185-8f18-dfc1eeeb310e
Melvin, Tracy
fd87f5eb-2bb9-48fa-b7be-7100ace9c50f

Braddick, Lucy M., Garland, Patrick J., Praeger, Matthew F., Butement, Jonathan, Friedrich, Daniel, Morgan, David J. and Melvin, Tracy (2012) Uniform aligned bioconjugation of biomolecule motifs for integration within microfabricated microfluidic devices. Analytical Biochemistry, 424 (2), 195-205. (doi:10.1016/j.ab.2012.02.020).

Record type: Article

Abstract

Full details and a step-by-step guide suitable for printing proteins aligned to micron-sized sensors and subsequent integration and alignment of microfluidic structures are presented. The precise alignment and grafting of micron-sized biomolecule patterns with an underlying substrate at predefined locations is achieved using a novel semi-automated microcontact printer. Through integration of optical alignment methods in the x, y, and z directions, uniform contact of micron-sized stamps is achieved. Feature compression of the stamp is avoided by fine control of the stamp during contact. This printing method has been developed in combination with robust, compatible bioconjugate chemistry for patterning of a dextran-functionalized silicon oxide substrate with a NeutrAvidin-"inked" stamp and subsequent incubation with a biotin-functionalized protein. The bioconjugate chemistry is such that uniform coverage of the protein (without denaturation) over the printed motif is obtained and reproduction of the initial mask shape and dimensions is achieved. Later integration with a microfluidic structure aligned with the printed motif on the substrate is also described.

Full text not available from this repository.

More information

e-pub ahead of print date: 25 February 2012
Published date: 15 May 2012
Keywords: microfluidic, biosensors, bioconjugation, bioaffinity sensors, lab-on-a-chip, microcontact printing, microfabrication
Organisations: Optoelectronics Research Centre

Identifiers

Local EPrints ID: 337288
URI: https://eprints.soton.ac.uk/id/eprint/337288
ISSN: 0003-2697
PURE UUID: 6d783454-b202-4ab4-ba75-6097e4206a1a

Catalogue record

Date deposited: 23 Apr 2012 10:33
Last modified: 06 Aug 2019 18:56

Export record

Altmetrics

Contributors

Author: Lucy M. Braddick
Author: Jonathan Butement
Author: Daniel Friedrich
Author: David J. Morgan
Author: Tracy Melvin

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of https://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×