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Uniform aligned bioconjugation of biomolecule motifs for integration within microfabricated microfluidic devices

Record type: Article

Full details and a step-by-step guide suitable for printing proteins aligned to micron-sized sensors and subsequent integration and alignment of microfluidic structures are presented. The precise alignment and grafting of micron-sized biomolecule patterns with an underlying substrate at predefined locations is achieved using a novel semi-automated microcontact printer. Through integration of optical alignment methods in the x, y, and z directions, uniform contact of micron-sized stamps is achieved. Feature compression of the stamp is avoided by fine control of the stamp during contact. This printing method has been developed in combination with robust, compatible bioconjugate chemistry for patterning of a dextran-functionalized silicon oxide substrate with a NeutrAvidin-"inked" stamp and subsequent incubation with a biotin-functionalized protein. The bioconjugate chemistry is such that uniform coverage of the protein (without denaturation) over the printed motif is obtained and reproduction of the initial mask shape and dimensions is achieved. Later integration with a microfluidic structure aligned with the printed motif on the substrate is also described.

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Citation

Braddick, Lucy M., Garland, Patrick J., Praeger, Matthew F., Butement, Jonathan, Friedrich, Daniel, Morgan, David J. and Melvin, Tracy (2012) Uniform aligned bioconjugation of biomolecule motifs for integration within microfabricated microfluidic devices Analytical Biochemistry, 424, (2), pp. 195-205. (doi:10.1016/j.ab.2012.02.020).

More information

e-pub ahead of print date: 25 February 2012
Published date: 15 May 2012
Keywords: microfluidic, biosensors, bioconjugation, bioaffinity sensors, lab-on-a-chip, microcontact printing, microfabrication
Organisations: Optoelectronics Research Centre

Identifiers

Local EPrints ID: 337288
URI: http://eprints.soton.ac.uk/id/eprint/337288
ISSN: 0003-2697
PURE UUID: 6d783454-b202-4ab4-ba75-6097e4206a1a

Catalogue record

Date deposited: 23 Apr 2012 10:33
Last modified: 18 Jul 2017 06:03

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Contributors

Author: Lucy M. Braddick
Author: Matthew F. Praeger
Author: Jonathan Butement
Author: Daniel Friedrich
Author: David J. Morgan
Author: Tracy Melvin

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