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The self-orientation of mammalian cells in optical tweezers - the importance of the nucleus

Perney, Nicolas M.B., Horak, Peter, Hanley, Neil A. and Melvin, Tracy (2012) The self-orientation of mammalian cells in optical tweezers - the importance of the nucleus Physical Biology, 9, (2), p. 24001. (doi:10.1088/1478-3975/9/2/024001). (PMID:22473086).

Record type: Article

Abstract

Here we present the first evidence showing that eukaryotic cells can be stably trapped in a single focused Gaussian beam with an orientation that is defined by the nucleus. A mammalian eukaryotic cell (in suspension) is trapped and is re-oriented in the focus of a linearly polarized Gaussian beam with a waist of dimension smaller than the radius of the nucleus. The cell reaches a position relative to the focus that is dictated by the nucleus and nuclear components. Our studies illustrate that the force exerted by the optical tweezers at locations within the cell can be predicted theoretically; the data obtained in this way is consistent with the experimental observations.

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More information

Published date: 3 April 2012
Keywords: instrumentation and measurement, medical physics, biological physics
Organisations: Optoelectronics Research Centre

Identifiers

Local EPrints ID: 337291
URI: http://eprints.soton.ac.uk/id/eprint/337291
ISSN: 1478-3967
PURE UUID: 8236f2a9-4906-430f-a125-6505bc680ec0
ORCID for Peter Horak: ORCID iD orcid.org/0000-0002-8710-8764

Catalogue record

Date deposited: 23 Apr 2012 13:41
Last modified: 18 Jul 2017 06:03

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Contributors

Author: Nicolas M.B. Perney
Author: Peter Horak ORCID iD
Author: Neil A. Hanley
Author: Tracy Melvin

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