Zhang, Yuxia, Yang, Zhihong, Whitby, Richard and Wang, Li
Regulation of miR-200c by nuclear receptors PPAR?, LRH-1 and SHP
Biochemical and Biophysical Research Communications, 416, (1-2), . (doi:10.1016/j.bbrc.2011.11.011). (PMID:22100809).
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We investigated regulation of miR-200c expression by nuclear receptors. Ectopic expression of miR-200c inhibited MHCC97H cell migration, which was abrogated by the synergistic effects of PPAR? and LRH-1 siRNAs. The expression of miR-200c was decreased by PPAR?/LRH-1 siRNAs and increased by SHP siRNAs, and overexpression of the receptors reversed the effects of their respective siRNAs. SHP siRNAs also drastically enhanced the ability of the LRH-1 agonist RJW100 to induce miR-200c and downregulate ZEB1 and ZEB2 proteins. Co-expression of PPAR? and LRH-1 moderately transactivated the miR-200c promoter, which was repressed by SHP co-expression. RJW100 caused strong activation of the miR-200c promoter. This is the first report to demonstrate that miR-200c expression is controlled by nuclear receptors.
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