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Regulation of miR-200c by nuclear receptors PPAR?, LRH-1 and SHP

Regulation of miR-200c by nuclear receptors PPAR?, LRH-1 and SHP
Regulation of miR-200c by nuclear receptors PPAR?, LRH-1 and SHP
We investigated regulation of miR-200c expression by nuclear receptors. Ectopic expression of miR-200c inhibited MHCC97H cell migration, which was abrogated by the synergistic effects of PPAR? and LRH-1 siRNAs. The expression of miR-200c was decreased by PPAR?/LRH-1 siRNAs and increased by SHP siRNAs, and overexpression of the receptors reversed the effects of their respective siRNAs. SHP siRNAs also drastically enhanced the ability of the LRH-1 agonist RJW100 to induce miR-200c and downregulate ZEB1 and ZEB2 proteins. Co-expression of PPAR? and LRH-1 moderately transactivated the miR-200c promoter, which was repressed by SHP co-expression. RJW100 caused strong activation of the miR-200c promoter. This is the first report to demonstrate that miR-200c expression is controlled by nuclear receptors.
nuclear receptor, microRNA, transcription, cell migration
0006-291X
135-139
Zhang, Yuxia
38d2c428-ad53-4907-8fbe-d26e495b79c4
Yang, Zhihong
e6e4733d-97db-4f87-aa0b-91db11901dc9
Whitby, Richard
45632236-ab00-4ad0-a02d-6209043e818b
Wang, Li
f54669eb-8e6b-43ea-a6df-47cda21d6950
Zhang, Yuxia
38d2c428-ad53-4907-8fbe-d26e495b79c4
Yang, Zhihong
e6e4733d-97db-4f87-aa0b-91db11901dc9
Whitby, Richard
45632236-ab00-4ad0-a02d-6209043e818b
Wang, Li
f54669eb-8e6b-43ea-a6df-47cda21d6950

Zhang, Yuxia, Yang, Zhihong, Whitby, Richard and Wang, Li (2011) Regulation of miR-200c by nuclear receptors PPAR?, LRH-1 and SHP. Biochemical and Biophysical Research Communications, 416 (1-2), 135-139. (doi:10.1016/j.bbrc.2011.11.011). (PMID:22100809)

Record type: Article

Abstract

We investigated regulation of miR-200c expression by nuclear receptors. Ectopic expression of miR-200c inhibited MHCC97H cell migration, which was abrogated by the synergistic effects of PPAR? and LRH-1 siRNAs. The expression of miR-200c was decreased by PPAR?/LRH-1 siRNAs and increased by SHP siRNAs, and overexpression of the receptors reversed the effects of their respective siRNAs. SHP siRNAs also drastically enhanced the ability of the LRH-1 agonist RJW100 to induce miR-200c and downregulate ZEB1 and ZEB2 proteins. Co-expression of PPAR? and LRH-1 moderately transactivated the miR-200c promoter, which was repressed by SHP co-expression. RJW100 caused strong activation of the miR-200c promoter. This is the first report to demonstrate that miR-200c expression is controlled by nuclear receptors.

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More information

Published date: 9 December 2011
Keywords: nuclear receptor, microRNA, transcription, cell migration
Organisations: Organic Chemistry: Synthesis, Catalysis and Flow, Chemistry

Identifiers

Local EPrints ID: 337315
URI: http://eprints.soton.ac.uk/id/eprint/337315
ISSN: 0006-291X
PURE UUID: 4e14f7bf-553f-4b18-8f75-31b197617ca5
ORCID for Richard Whitby: ORCID iD orcid.org/0000-0002-9891-5502

Catalogue record

Date deposited: 24 Apr 2012 09:27
Last modified: 19 Nov 2019 02:04

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