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Neisseria gonorrhoeae pilus attenuates cytokine response of human fallopian tube explants

Record type: Article

Background. A role for pilus during attachment of Neisseria gonorrhoeae to epithelia of the female reproductive tract is currently assumed. However, Pil(-) gonococci have been observed during infection of the reproductive tract, which prompted us to examine the effect of pili on the dynamics of infection and the inflammatory responses of mucosal explants of the human Fallopian tube.
Methods. Mucosal explants were infected in vitro with Opa negative Pil(-) and Pil(+) N. gonorrhoeae strains.
Results. Piliation enhanced gonococcal adherence to the epithelium within 3 h of infection (P < 0.05) but thereafter did not offer advantage to gonococci to colonize the epithelial cell surface (P > 0.05). No differences were found between the strains in numbers of gonococci inside epithelial cells. Pil(-) bacteria induced higher levels (P < 0.05) of IL-1 beta, TNF-alpha, GM-CSF, MCP-1, and MIP-1 beta than Pil(+) bacteria. There were no differences between both strains in LOS pattern, and Pil expression did not change after coincubation with mucosal strips.
Conclusions. Results show that gonococcal invasion of the human Fallopian tube can occur independently of pilus or Opa expression, and suggest that pilus, by inhibition of several key elements of the initial inflammatory response, facilitates sustained infection of this organ.

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Citation

Velasquez, Luis, Garcia, Katherine, Morales, Francisco, Heckels, John E., Orihuela, Pedro, Rodas, Paula I., Christodoulides, Myron and Cardenas, Hugo (2012) Neisseria gonorrhoeae pilus attenuates cytokine response of human fallopian tube explants Journal of Biomedicine and Biotechnology, 2012, 491298-[7pp]. (doi:10.1155/2012/491298). (PMID:22318778).

More information

Published date: 24 January 2012
Organisations: Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 337363
URI: http://eprints.soton.ac.uk/id/eprint/337363
ISSN: 1110-7243
PURE UUID: b7ae0bd4-a705-4f81-a52a-31a195dd4258

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Date deposited: 25 Apr 2012 09:13
Last modified: 18 Jul 2017 06:03

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Contributors

Author: Luis Velasquez
Author: Katherine Garcia
Author: Francisco Morales
Author: John E. Heckels
Author: Pedro Orihuela
Author: Paula I. Rodas
Author: Hugo Cardenas

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