Coadministration of the cyanobacterial lipopolysaccharide antagonist CyP with antibiotic inhibits cytokine production by an in vitro meningitis model infected with Neisseria meningitidis

Oliver, Rebecca, Staples, Karl J., Heckels, John, Rossetti, Carlo, Molteni, Monica and Christodoulides, Myron (2012) Coadministration of the cyanobacterial lipopolysaccharide antagonist CyP with antibiotic inhibits cytokine production by an in vitro meningitis model infected with Neisseria meningitidis. Journal of Antimicrobial Chemotherapy, 67 (5), 1145-1154. (doi:10.1093/jac/dks031). (PMID:22334603)

Abstract

In this study, the objective was to determine the anti-inflammatory properties of CyP, a cyanobacterial lipopolysaccharide (LPS) antagonist, used in combination with antibiotic chemotherapy during infection of an in vitro meningitis model infected with Neisseria meningitidis (meningococcus). Monocultures of human meningioma cells and meningioma-primary human macrophage co-cultures were infected with meningococci (10(2)-10(8) cfu/monolayer) or treated with isolated outer membranes or purified LPS (0.1-100 ng/monolayer) from N. meningitidis. CyP (1-20 mug/monolayer) was added at intervals from t?=?0 to 4 h, with and without benzylpenicillin (1-20 mug/monolayer). The antagonistic effect of CyP and its adjunctive properties to benzylpenicillin administration was determined by measuring cytokine levels in culture supernatants after 24 h. CyP significantly inhibited (P?<?0.05) the secretion of interleukin (IL)-6, IL-8, monocyte chemoattractant protein (MCP)-1 and RANTES ('regulated upon activation, normal T cell expressed and secreted') (overall reduction levels from 50% to >95%) by meningioma cell lines and meningioma-macrophage co-cultures challenged with either live meningococci or bacterial components. Inhibition was effective when CyP was added within 2 h of challenge (P?<?0.05) and was still pronounced by 4 h. In the co-culture model, CyP alone partially inhibited IL-1beta secretion, but did not prevent tumour necrosis factor (TNF)-alpha secretion, whereas penicillin alone inhibited IL-1beta and TNF-alpha but conversely did not reduce MCP-1 and RANTES secretion. However, coadministration of CyP and penicillin in both models had an additive effect and restored the overall inhibitory profile. CyP inhibits cytokine production in an in vitro meningitis model and augments the anti-inflammatory response when combined with benzylpenicillin. Administration of an LPS antagonist with antibiotic merits consideration in the emergency treatment of patients presenting with meningococcal infection.

This record has no associated files available for download.

Contributors

Author: Karl J. Staples

Author: Myron Christodoulides

Download statistics