The University of Southampton
University of Southampton Institutional Repository

Airway epithelial transcription factor NK2 homeobox 1 inhibits mucous cell metaplasia and Th2 inflammation

Maeda, Yutaka, Chen, Gang, Xu, Yan, Haitchi, Hans Michael, Du, Lingling, Keiser, Angela R., Howarth, Peter H, Davies, Donna E., Holgate, Stephen T. and Whitsett, Jeffrey A. (2011) Airway epithelial transcription factor NK2 homeobox 1 inhibits mucous cell metaplasia and Th2 inflammation American Journal of Respiratory and Critical Care Medicine, 184, (4), pp. 421-429. (doi:10.1164/rccm.201101-0106OC). (PMID:21562130).

Record type: Article


Rationale: Airway mucous cell metaplasia and chronic inflammation are pathophysiological features that influence morbidity and mortality associated with asthma and other chronic pulmonary disorders. Elucidation of the molecular mechanisms regulating mucous metaplasia and hypersecretion provides the scientific basis for diagnostic and therapeutic opportunities to improve the care of chronic pulmonary diseases.

Objectives: To determine the role of the airway epithelial–specific transcription factor NK2 homeobox 1 (NKX2-1, also known as thyroid transcription factor-1 [TTF-1]) in mucous cell metaplasia and lung inflammation.

Methods: Expression of NKX2-1 in airway epithelial cells from patients with asthma was analyzed. NKX2-1+/? gene targeted or transgenic mice expressing NKX2-1 in conducting airway epithelial cells were sensitized to the aeroallergen ovalbumin. In vitro studies were used to identify mechanisms by which NKX2-1 regulates mucous cell metaplasia and inflammation.

Measurements and Main Results: NKX2-1 was suppressed in airway epithelial cells from patients with asthma. Reduced expression of NKX2-1 in heterozygous NKX2-1+/? gene targeted mice increased mucous metaplasia in the small airways after pulmonary sensitization to ovalbumin. Conversely, mucous cell metaplasia induced by aeroallergen was inhibited by expression of NKX2-1 in the respiratory epithelium in vivo. Genome-wide mRNA analysis of lung tissue from ovalbumin-treated mice demonstrated that NKX2-1 inhibited mRNAs associated with mucous metaplasia and Th2-regulated inflammation, including Spdef, Ccl17, and Il13. In vitro, NKX2-1 inhibited SPDEF, a critical regulator of airway mucous cell metaplasia, and the Th2 chemokine CCL26.

Conclusions: The present data demonstrate a novel function for NKX2-1 in a gene network regulating mucous cell metaplasia and allergic inflammation in the respiratory epithelium.

Full text not available from this repository.

More information

e-pub ahead of print date: 11 May 2011
Published date: 15 August 2011
Keywords: asthma, goblet cell, respiratory epithelium, NK2 homeobox 1
Organisations: Clinical & Experimental Sciences


Local EPrints ID: 337444
ISSN: 1073-449X
PURE UUID: 0712dbf3-00b8-4fd2-b001-76af673829bd

Catalogue record

Date deposited: 26 Apr 2012 11:21
Last modified: 18 Jul 2017 06:03

Export record



Author: Yutaka Maeda
Author: Gang Chen
Author: Yan Xu
Author: Hans Michael Haitchi
Author: Lingling Du
Author: Angela R. Keiser
Author: Peter H Howarth
Author: Donna E. Davies
Author: Jeffrey A. Whitsett

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton:

ePrints Soton supports OAI 2.0 with a base URL of

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.