A role for sigma factor ?(E) in Corynebacterium pseudotuberculosis resistance to nitric oxide/peroxide stress
A role for sigma factor ?(E) in Corynebacterium pseudotuberculosis resistance to nitric oxide/peroxide stress
Pathogenic intracellular bacteria can respond to antimicrobial mechanisms of the host cell through transient activation of stress-responsive genes by alternative sigma (?) factors of the RNA polymerase. We evaluated the contribution of the extracytoplasmic function sigma factor ?(E) for Corynebacterium pseudotuberculosis resistance to stress conditions resembling those found intracellularly during infection. A sigE-null mutant strain (?sigE) of this bacterium was more susceptible in vitro to acidic pH, cell surface stressors, and biologically relevant concentrations of nitric oxide (NO). The same mutant strain was unable to persist in C57BL/6 mice but remained infective in mice lacking inducible nitric oxide synthase (iNOS), confirming the significance of ?(E) for resistance to nitric oxide/peroxide stress in vivo. High-throughput proteomic analysis identified NO-responsive extracellular proteins of C. pseudotuberculosis and demonstrated the participation of ?(E) in composition of this bacterium's exoproteome.
corynebacterium pseudotuberculosis, sigma factor, nitric oxide, inducible nitric oxide synthase
Pacheco, Luis G.C.
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Castro, Thiago L.P.
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Carvalho, Rodrigo D.
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Moraes, Pablo M.
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Dorella, Fernanda A.
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Carvalho, Natália B.
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Slade, Susan E.
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Scrivens, James H.
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Feelisch, Martin
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Meyer, Roberto
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Miyoshi, Anderson
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Oliveira, Sergio C.
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Dowson, Christopher G
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Azevedo, Vasco
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3 April 2012
Pacheco, Luis G.C.
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Castro, Thiago L.P.
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Carvalho, Rodrigo D.
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Moraes, Pablo M.
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Dorella, Fernanda A.
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Carvalho, Natália B.
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Slade, Susan E.
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Scrivens, James H.
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Feelisch, Martin
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Meyer, Roberto
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Miyoshi, Anderson
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Oliveira, Sergio C.
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Dowson, Christopher G
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Azevedo, Vasco
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Pacheco, Luis G.C., Castro, Thiago L.P., Carvalho, Rodrigo D., Moraes, Pablo M., Dorella, Fernanda A., Carvalho, Natália B., Slade, Susan E., Scrivens, James H., Feelisch, Martin, Meyer, Roberto, Miyoshi, Anderson, Oliveira, Sergio C., Dowson, Christopher G and Azevedo, Vasco
(2012)
A role for sigma factor ?(E) in Corynebacterium pseudotuberculosis resistance to nitric oxide/peroxide stress.
Frontiers in Microbiology, 3 (126).
(doi:10.3389/fmicb.2012.00126).
(PMID:22514549)
Abstract
Pathogenic intracellular bacteria can respond to antimicrobial mechanisms of the host cell through transient activation of stress-responsive genes by alternative sigma (?) factors of the RNA polymerase. We evaluated the contribution of the extracytoplasmic function sigma factor ?(E) for Corynebacterium pseudotuberculosis resistance to stress conditions resembling those found intracellularly during infection. A sigE-null mutant strain (?sigE) of this bacterium was more susceptible in vitro to acidic pH, cell surface stressors, and biologically relevant concentrations of nitric oxide (NO). The same mutant strain was unable to persist in C57BL/6 mice but remained infective in mice lacking inducible nitric oxide synthase (iNOS), confirming the significance of ?(E) for resistance to nitric oxide/peroxide stress in vivo. High-throughput proteomic analysis identified NO-responsive extracellular proteins of C. pseudotuberculosis and demonstrated the participation of ?(E) in composition of this bacterium's exoproteome.
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Published date: 3 April 2012
Keywords:
corynebacterium pseudotuberculosis, sigma factor, nitric oxide, inducible nitric oxide synthase
Organisations:
Clinical & Experimental Sciences
Identifiers
Local EPrints ID: 337690
URI: http://eprints.soton.ac.uk/id/eprint/337690
ISSN: 1664-302X
PURE UUID: 35457a96-f4ad-49be-ac13-949636727600
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Date deposited: 02 May 2012 11:56
Last modified: 15 Mar 2024 03:41
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Contributors
Author:
Luis G.C. Pacheco
Author:
Thiago L.P. Castro
Author:
Rodrigo D. Carvalho
Author:
Pablo M. Moraes
Author:
Fernanda A. Dorella
Author:
Natália B. Carvalho
Author:
Susan E. Slade
Author:
James H. Scrivens
Author:
Roberto Meyer
Author:
Anderson Miyoshi
Author:
Sergio C. Oliveira
Author:
Christopher G Dowson
Author:
Vasco Azevedo
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