The role of vascular myoglobin in nitrite-mediated blood vessel relaxation

Ormerod, Julian O.M., Ashrafian, Houman, Maher, Abdul R., Arif, Sayqa, Steeples, Violetta, Born, Gustav V.R., Egginton, Stuart, Feelisch, Martin, Watkins, Hugh and Frenneaux, Michael P. (2010) The role of vascular myoglobin in nitrite-mediated blood vessel relaxation Cardiovascular Research, 89, (3), pp. 560-565. (doi:10.1093/cvr/cvq299). (PMID:20889759).


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Aims: this work investigates the role of myoglobin in mediating the vascular relaxation induced by nitrite. Nitrite, previously considered an inert by-product of nitric oxide metabolism, is now believed to play an important role in several areas of pharmacology and physiology. Myoglobin can act as a nitrite reductase in the heart, where it is plentiful, but it is present at a far lower level in vascular smooth muscle—indeed, its existence in the vessel wall is controversial. Haem proteins have been postulated to be important in nitrite-induced vasodilation, but the specific role of myoglobin is unknown. The current study was designed to confirm the presence of myoglobin in murine aortic tissue and to test the hypothesis that vascular wall myoglobin is important for nitrite-induced vasodilation.

Methods and results: aortic rings from wild-type and myoglobin knockout mice were challenged with nitrite, before and after exposure to the haem-protein inhibitor carbon monoxide (CO). CO inhibited vasodilation in wild-type rings but not in myoglobin-deficient rings. Restitution of myoglobin using a genetically modified adenovirus both increased vasodilation to nitrite and reinstated the wild-type pattern of response to CO.

Conclusion: myoglobin is present in the murine vasculature and contributes significantly to nitrite-induced vasodilation

Item Type: Article
Digital Object Identifier (DOI): doi:10.1093/cvr/cvq299
ISSNs: 0008-6363 (print)
Organisations: Clinical & Experimental Sciences
ePrint ID: 337694
Date :
Date Event
1 October 2010e-pub ahead of print
15 February 2011Published
Date Deposited: 02 May 2012 11:47
Last Modified: 17 Apr 2017 17:14
Further Information:Google Scholar

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