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Interleukin-1beta does not affect the energy metabolism of rat organotypic hippocampal-slice cultures

Interleukin-1beta does not affect the energy metabolism of rat organotypic hippocampal-slice cultures
Interleukin-1beta does not affect the energy metabolism of rat organotypic hippocampal-slice cultures
The aim of this study was to examine the effect of the archetypal pro-inflammatory cytokine, interleukin-1beta (IL-1?), on high-energy phosphate levels within an ex vivo rat organotypic hippocampal-slice culture (OHSC) preparation using phosphorus ((31)P) magnetic resonance spectroscopy (MRS). Intrastriatal microinjection of IL-1? induces a chronic reduction in the apparent diffusion coefficient (ADC) of tissue water, which may be indicative of metabolic failure as established by in vivo models of acute cerebral ischaemia. The OHSC preparation enables examination of the effects of IL-1? on brain parenchyma per se, independent of the potentially confounding effects encountered in vivo such as perfusion changes, blood-brain barrier (BBB) breakdown and leukocyte recruitment. (31)P MRS is a technique that can detect multiple high-energy phosphate metabolites within a sample non-invasively. Here, for the first time, we characterise the energy metabolism of OHSCs using (31)P MRS and demonstrate that IL-1? does not compromise high-energy phosphate metabolism. Thus, the chronic reduction in ADC observed in vivo is unlikely to be a consequence of metabolic failure.
0304-3940
114-118
Saggu, Raman
89f1f89b-c536-4965-8af3-d16837ee3c32
Morrison, Barclay
97d2b4dc-0ce1-4706-a0ee-bb3ce7733f22
Lowe, John P.
d08a6bff-c43e-488f-a072-eaa5873c4cd7
Pringle, Ashley K.
6339ed95-c491-43a8-b2fb-2384466dc80d
Saggu, Raman
89f1f89b-c536-4965-8af3-d16837ee3c32
Morrison, Barclay
97d2b4dc-0ce1-4706-a0ee-bb3ce7733f22
Lowe, John P.
d08a6bff-c43e-488f-a072-eaa5873c4cd7
Pringle, Ashley K.
6339ed95-c491-43a8-b2fb-2384466dc80d

Saggu, Raman, Morrison, Barclay, Lowe, John P. and Pringle, Ashley K. (2012) Interleukin-1beta does not affect the energy metabolism of rat organotypic hippocampal-slice cultures. Neuroscience Letters, 508 (2), 114-118. (doi:10.1016/j.neulet.2011.12.032). (PMID:22215116)

Record type: Article

Abstract

The aim of this study was to examine the effect of the archetypal pro-inflammatory cytokine, interleukin-1beta (IL-1?), on high-energy phosphate levels within an ex vivo rat organotypic hippocampal-slice culture (OHSC) preparation using phosphorus ((31)P) magnetic resonance spectroscopy (MRS). Intrastriatal microinjection of IL-1? induces a chronic reduction in the apparent diffusion coefficient (ADC) of tissue water, which may be indicative of metabolic failure as established by in vivo models of acute cerebral ischaemia. The OHSC preparation enables examination of the effects of IL-1? on brain parenchyma per se, independent of the potentially confounding effects encountered in vivo such as perfusion changes, blood-brain barrier (BBB) breakdown and leukocyte recruitment. (31)P MRS is a technique that can detect multiple high-energy phosphate metabolites within a sample non-invasively. Here, for the first time, we characterise the energy metabolism of OHSCs using (31)P MRS and demonstrate that IL-1? does not compromise high-energy phosphate metabolism. Thus, the chronic reduction in ADC observed in vivo is unlikely to be a consequence of metabolic failure.

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More information

e-pub ahead of print date: 27 December 2011
Published date: 6 February 2012
Organisations: Faculty of Medicine

Identifiers

Local EPrints ID: 337747
URI: http://eprints.soton.ac.uk/id/eprint/337747
ISSN: 0304-3940
PURE UUID: f5175de8-f484-48b8-ab3c-e12cc94d0185
ORCID for Ashley K. Pringle: ORCID iD orcid.org/0000-0003-2421-4380

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Date deposited: 03 May 2012 12:51
Last modified: 20 Jul 2019 01:19

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Contributors

Author: Raman Saggu
Author: Barclay Morrison
Author: John P. Lowe

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