Circulating NO pool: assessment of nitrite and nitroso species in blood and tissues
Circulating NO pool: assessment of nitrite and nitroso species in blood and tissues
The formation of nitric oxide (NO) has been linked to many regulatory functions in mammalian cells. With the appreciation that NO-mediated nitrosation reactions are involved in cell signaling and pathology there is a need to elucidate and better characterize the different biochemical pathways of NO in vivo. Despite significant methodological advances over the years one major obstacle in assessing the significance of nitrosated species and other NO-related metabolites remains: their reliable measurement in complex biological matrices. In this review we briefly discuss the major routes of NO metabolism and transport in the mammalian circulation, considering plasma, red blood cell, and tissue compartments separately. In addition, we attempt to give a recommendation as to the most appropriate analytical technique and sample processing procedures for the reliable quantification of either species.
413-422
Rassaf, Tienush
a820a375-219a-4fa2-ae10-e77f4b1eb37c
Feelisch, Martin
8c1b9965-8614-4e85-b2c6-458a2e17eafd
Kelm, Malte
db2bb062-32d7-4b50-9f65-8ba89ffa5f42
15 February 2004
Rassaf, Tienush
a820a375-219a-4fa2-ae10-e77f4b1eb37c
Feelisch, Martin
8c1b9965-8614-4e85-b2c6-458a2e17eafd
Kelm, Malte
db2bb062-32d7-4b50-9f65-8ba89ffa5f42
Abstract
The formation of nitric oxide (NO) has been linked to many regulatory functions in mammalian cells. With the appreciation that NO-mediated nitrosation reactions are involved in cell signaling and pathology there is a need to elucidate and better characterize the different biochemical pathways of NO in vivo. Despite significant methodological advances over the years one major obstacle in assessing the significance of nitrosated species and other NO-related metabolites remains: their reliable measurement in complex biological matrices. In this review we briefly discuss the major routes of NO metabolism and transport in the mammalian circulation, considering plasma, red blood cell, and tissue compartments separately. In addition, we attempt to give a recommendation as to the most appropriate analytical technique and sample processing procedures for the reliable quantification of either species.
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e-pub ahead of print date: 19 December 2003
Published date: 15 February 2004
Organisations:
Clinical & Experimental Sciences
Identifiers
Local EPrints ID: 337848
URI: http://eprints.soton.ac.uk/id/eprint/337848
ISSN: 0891-5849
PURE UUID: 45511c8f-9825-42a1-9b16-bcf4f5f391d0
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Date deposited: 22 Jun 2012 09:30
Last modified: 15 Mar 2024 03:41
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Author:
Tienush Rassaf
Author:
Malte Kelm
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