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Comparison of the reactivity of nitric oxide and nitroxyl with heme proteins. A chemical discussion of the differential biological effects of these redox related products of NOS

Miranda, Katrina M., Nims, Raymond W., Thomas, Douglas D., Espey, Michael G., Citrin, Deborah, Bartberger, Michael D., Paolocci, Nazareno, Fukuto, Jon M., Feelisch, Martin and Wink, David A. (2003) Comparison of the reactivity of nitric oxide and nitroxyl with heme proteins. A chemical discussion of the differential biological effects of these redox related products of NOS Journal of Inorganic Biochemistry, 93, (1-2), pp. 52-60. (doi:10.1016/S0162-0134(02)00498-1). (PMID:12538052).

Record type: Article

Abstract

Investigations on the biological effects of nitric oxide (NO) derived from nitric oxide synthase (NOS) have led to an explosion in biomedical research over the last decade. The chemistry of this diatomic radical is key to its biological effects. Recently, nitroxyl (HNO/NO(-)) has been proposed to be another important constituent of NO biology. However, these redox siblings often exhibit orthogonal behavior in physiological and cellular responses. We therefore explored the chemistry of NO and HNO with heme proteins in different redox states and observed that HNO favors reaction with ferric heme while NO favors ferrous, consistent with previous reports. Further results show that HNO and NO were equally effective in inhibiting cytochrome P450 activity, which involves ferric and ferrous complexes. The differential chemical behavior of NO and HNO toward heme proteins provides insight into mechanisms of activity that not only helps explain some of the opposing effects observed in NOS-mediated events, but offers a unique control mechanism for the biological action of NO.

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More information

Published date: 1 January 2003
Keywords: nitric oxide, nitroxyl, angeli’s salt, heme, horseradish peroxidase, myoglobin, hemoglobin
Organisations: Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 337858
URI: http://eprints.soton.ac.uk/id/eprint/337858
ISSN: 0162-0134
PURE UUID: 93d60dd5-6208-4cc6-bc7c-adea4061e3b2
ORCID for Martin Feelisch: ORCID iD orcid.org/0000-0003-2320-1158

Catalogue record

Date deposited: 22 Jun 2012 10:48
Last modified: 18 Jul 2017 06:00

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Contributors

Author: Katrina M. Miranda
Author: Raymond W. Nims
Author: Douglas D. Thomas
Author: Michael G. Espey
Author: Deborah Citrin
Author: Michael D. Bartberger
Author: Nazareno Paolocci
Author: Jon M. Fukuto
Author: Martin Feelisch ORCID iD
Author: David A. Wink

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