The University of Southampton
University of Southampton Institutional Repository

Continuous exposure to high concentrations of nitric oxide leads to persistent inhibition of oxygen consumption by J774 cells as well as extraction of oxygen by the extracellular medium

Record type: Article

Nitric oxide (NO) plays a key role in many physiological and pathophysiological events, including the control of cell respiration. Both reversible and irreversible inhibition of mitochondrial respiration have been reported following the generation of NO by cells. We have exposed the murine macrophage cell line J774 to high concentrations of NO, such as are generated in some pathological conditions, and determined their effect on oxygen consumption. We observed a persistent inhibition of respiration which was due to a redox-dependent, progressive inhibition of complex I activity. No other enzyme of the respiratory chain was inhibited in this way. At the same time, we detected a paradoxical removal of oxygen by the extracellular medium. This removal was due to a chemical interaction between dissolved oxygen and NO-related species released from cells exposed to NO. A similar removal of oxygen by the cell supernatant also occurred following activation of cells with cytokines and bacterial products. Thus, the amounts of NO generated during pathological conditions may contribute to tissue hypoxia both by inhibiting cell respiration and by promoting removal of oxygen from the extracellular medium.

Full text not available from this repository.

Citation

Orsi, Antonia, Beltrán, Belén, Clementi, Emilio, Hallén, Katarina, Feelisch, Martin and Moncada, Salvador (2000) Continuous exposure to high concentrations of nitric oxide leads to persistent inhibition of oxygen consumption by J774 cells as well as extraction of oxygen by the extracellular medium Biochemical Journal, 346, pp. 407-412. (PMID:10677360).

More information

Published date: 1 March 2000
Keywords: cell respiration, complex I, glutathione, nitric oxide, S-nitrosation
Organisations: Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 337876
URI: http://eprints.soton.ac.uk/id/eprint/337876
ISSN: 1470-8728
PURE UUID: 8211a8fc-6379-4bb1-bcca-5f181d331f32
ORCID for Martin Feelisch: ORCID iD orcid.org/0000-0003-2320-1158

Catalogue record

Date deposited: 29 Jun 2012 10:37
Last modified: 18 Jul 2017 06:00

Export record

Contributors

Author: Antonia Orsi
Author: Belén Beltrán
Author: Emilio Clementi
Author: Katarina Hallén
Author: Martin Feelisch ORCID iD
Author: Salvador Moncada

University divisions


Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×