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Opposite effects of nitric oxide and nitroxyl on postischemic myocardial injury

Ma, Xin L., Gao, Feng, Liu, Gao-Lin, Lopez, Bernard L., Christopher, Theodore A., Fukuto, Jon M., Wink, David A. and Feelisch, Martin (1999) Opposite effects of nitric oxide and nitroxyl on postischemic myocardial injury Proceedings of the National Academy of Sciences, 96, (25), pp. 14617-14622. (doi:10.1073/pnas.96.25.14617). (PMID:10588754).

Record type: Article

Abstract

Recent experimental evidence suggests that reactive nitrogen oxide species can contribute significantly to postischemic myocardial injury. The aim of the present study was to evaluate the role of two reactive nitrogen oxide species, nitroxyl (NO(-)) and nitric oxide (NO(.)), in myocardial ischemia and reperfusion injury. Rabbits were subjected to 45 min of regional myocardial ischemia followed by 180 min of reperfusion. Vehicle (0.9% NaCl), 1 micromol/kg S-nitrosoglutathione (GSNO) (an NO(.) donor), or 3 micromol/kg Angeli's salt (AS) (a source of NO(-)) were given i.v. 5 min before reperfusion. Treatment with GSNO markedly attenuated reperfusion injury, as evidenced by improved cardiac function, decreased plasma creatine kinase activity, reduced necrotic size, and decreased myocardial myeloperoxidase activity. In contrast, the administration of AS at a hemodynamically equieffective dose not only failed to attenuate but, rather, aggravated reperfusion injury, indicated by an increased left ventricular end diastolic pressure, myocardial creatine kinase release and necrotic size. Decomposed AS was without effect. Co-administration of AS with ferricyanide, a one-electron oxidant that converts NO(-) to NO(.), completely blocked the injurious effects of AS and exerted significant cardioprotective effects similar to those of GSNO. These results demonstrate that, although NO(.) is protective, NO(-) increases the tissue damage that occurs during ischemia/reperfusion and suggest that formation of nitroxyl may contribute to postischemic myocardial injury.

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Published date: 7 December 1999
Organisations: Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 337879
URI: http://eprints.soton.ac.uk/id/eprint/337879
ISSN: 0027-8424
PURE UUID: 0621e290-a96c-46aa-a695-824af0a79956
ORCID for Martin Feelisch: ORCID iD orcid.org/0000-0003-2320-1158

Catalogue record

Date deposited: 29 Jun 2012 11:01
Last modified: 18 Jul 2017 06:00

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Contributors

Author: Xin L. Ma
Author: Feng Gao
Author: Gao-Lin Liu
Author: Bernard L. Lopez
Author: Theodore A. Christopher
Author: Jon M. Fukuto
Author: David A. Wink
Author: Martin Feelisch ORCID iD

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