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Effect of nitric oxide donors on neointima formation and vascular reactivity in the collared carotid artery of rabbits

De Meyer, Guido R., Bult, Hidde, Ustünes, Levent, Kockx, Mark M., Feelisch, Martin and Herman, Arnold G. (1995) Effect of nitric oxide donors on neointima formation and vascular reactivity in the collared carotid artery of rabbits Journal of Cardiovascular Pharmacology, 26, (2), pp. 272-279. (PMID:7475052).

Record type: Article


Intimal thickening in arteries is considered a site of predilection for atherosclerosis. We investigated whether oral application of the nitric oxide (NO) donors SPM-5185 [N-nitratopivaloyl-S-(N'-acetylalanyl)-cysteine ethylester, 10 mg/kg body weight twice daily (b.i.d.)] and molsidomine (10 mg/kg body weight/day) can retard neointima formation and changes in vascular reactivity induced by a nonocclusive, soft silicone collar positioned around the left carotid artery of rabbits. The contralateral carotid artery was sham operated and served as a control. Drug and placebo (diet without drug) treatments were initiated 7 days before placement of the collar. At the end of the experiments, two segments were cut from each collared and sham-treated artery, one for measurement of the cross-sectional area of intima and media and the other for isometric tension recording. Sham treatment did not result in intimal thickening in either group. In contrast, the intima/media (I/M) ratio was considerably increased after 14 days of collar treatment as a result of neointima formation. Intimal thickening was significantly inhibited by SPM-5185 (I/M ratio 0.05 +/- 0.01 vs. 0.11 +/- 0.02, p < 0.05), but not by molsidomine (0.06 +/- 0.02 vs. 0.08 +/- 0.02, p = 0.49), which is a donor of both NO and superoxide anions. Neither collar nor NO donor treatment altered the area of the media. SPM-5185 did not alter the percentage of replicating smooth muscle cells (SMC) in the media after collar treatment, as demonstrated by their immunoreactivity for proliferating cell nuclear antigen (PCNA).(ABSTRACT TRUNCATED AT 250 WORDS)

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Published date: August 1995
Organisations: Clinical & Experimental Sciences


Local EPrints ID: 337899
ISSN: 0160-2446
PURE UUID: 172f7069-dadc-44e2-8ca8-803cf90f9094
ORCID for Martin Feelisch: ORCID iD

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Date deposited: 29 Jun 2012 15:49
Last modified: 18 Jul 2017 06:00

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Author: Guido R. De Meyer
Author: Hidde Bult
Author: Levent Ustünes
Author: Mark M. Kockx
Author: Martin Feelisch ORCID iD
Author: Arnold G. Herman

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