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Bioassay discrimination between nitric oxide (NO) and nitroxyl (NO-) using L-cysteine

Record type: Article

Nitroxyl (NO-) is the one-electron reduction product of nitric oxide (NO.). Recently, NO- generating compounds were shown to possess potent vasorelaxant activity and this was attributed to the ready conversion of NO- to NO.. Because of its metastable character, direct chemical detection of NO- or its conjugated acid, HNO, has not been accomplished yet. In order to gain further insight into the cellular mode of action of NO- generating compounds we aimed at finding a means to discriminate NO- from NO. by bioassay. Using isolated rat aortic rings in organ baths, we here show that high concentrations of L-cysteine cause complete inhibition of the vasorelaxant response to NO- (generated from Angeli's salt and sodium nitroxyl) whereas responses to authentic NO. and S-nitrosocysteine are largely enhanced. Preliminary results indicate that the inhibition by L-cysteine of NO- activity may be mediated in part by enzymatic and non-enzymatic mechanisms. Whether or not NO- generating compounds will have promising therapeutic potential as a new class of NO.- donors will not least depend on their interference with enzymatic routes susceptible to inhibition by NO-.

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Citation

Pino, R.Z. and Feelisch, M. (1994) Bioassay discrimination between nitric oxide (NO) and nitroxyl (NO-) using L-cysteine Biochemical and Biophysical Research Communications, 201, (1), pp. 54-62. (doi:10.1006/bbrc.1994.1668). (PMID:8198612).

More information

Published date: 30 May 1994
Organisations: Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 337906
URI: http://eprints.soton.ac.uk/id/eprint/337906
ISSN: 0006-291X
PURE UUID: 4e708a7f-1d94-4712-8934-1bcb3260afae
ORCID for M. Feelisch: ORCID iD orcid.org/0000-0003-2320-1158

Catalogue record

Date deposited: 15 Aug 2012 10:22
Last modified: 18 Jul 2017 06:00

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Contributors

Author: R.Z. Pino
Author: M. Feelisch ORCID iD

University divisions

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