Bioassay discrimination between nitric oxide (NO) and nitroxyl (NO-) using L-cysteine
Bioassay discrimination between nitric oxide (NO) and nitroxyl (NO-) using L-cysteine
Nitroxyl (NO-) is the one-electron reduction product of nitric oxide (NO.). Recently, NO- generating compounds were shown to possess potent vasorelaxant activity and this was attributed to the ready conversion of NO- to NO.. Because of its metastable character, direct chemical detection of NO- or its conjugated acid, HNO, has not been accomplished yet. In order to gain further insight into the cellular mode of action of NO- generating compounds we aimed at finding a means to discriminate NO- from NO. by bioassay. Using isolated rat aortic rings in organ baths, we here show that high concentrations of L-cysteine cause complete inhibition of the vasorelaxant response to NO- (generated from Angeli's salt and sodium nitroxyl) whereas responses to authentic NO. and S-nitrosocysteine are largely enhanced. Preliminary results indicate that the inhibition by L-cysteine of NO- activity may be mediated in part by enzymatic and non-enzymatic mechanisms. Whether or not NO- generating compounds will have promising therapeutic potential as a new class of NO.- donors will not least depend on their interference with enzymatic routes susceptible to inhibition by NO-.
54-62
Pino, R.Z.
b9e0937f-d082-46b7-b387-a9cabb4d03e4
Feelisch, M.
8c1b9965-8614-4e85-b2c6-458a2e17eafd
30 May 1994
Pino, R.Z.
b9e0937f-d082-46b7-b387-a9cabb4d03e4
Feelisch, M.
8c1b9965-8614-4e85-b2c6-458a2e17eafd
Pino, R.Z. and Feelisch, M.
(1994)
Bioassay discrimination between nitric oxide (NO) and nitroxyl (NO-) using L-cysteine.
Biochemical and Biophysical Research Communications, 201 (1), .
(doi:10.1006/bbrc.1994.1668).
(PMID:8198612)
Abstract
Nitroxyl (NO-) is the one-electron reduction product of nitric oxide (NO.). Recently, NO- generating compounds were shown to possess potent vasorelaxant activity and this was attributed to the ready conversion of NO- to NO.. Because of its metastable character, direct chemical detection of NO- or its conjugated acid, HNO, has not been accomplished yet. In order to gain further insight into the cellular mode of action of NO- generating compounds we aimed at finding a means to discriminate NO- from NO. by bioassay. Using isolated rat aortic rings in organ baths, we here show that high concentrations of L-cysteine cause complete inhibition of the vasorelaxant response to NO- (generated from Angeli's salt and sodium nitroxyl) whereas responses to authentic NO. and S-nitrosocysteine are largely enhanced. Preliminary results indicate that the inhibition by L-cysteine of NO- activity may be mediated in part by enzymatic and non-enzymatic mechanisms. Whether or not NO- generating compounds will have promising therapeutic potential as a new class of NO.- donors will not least depend on their interference with enzymatic routes susceptible to inhibition by NO-.
Other
1994 Pino-BBRC.PDF
- Other
More information
Published date: 30 May 1994
Organisations:
Clinical & Experimental Sciences
Identifiers
Local EPrints ID: 337906
URI: http://eprints.soton.ac.uk/id/eprint/337906
ISSN: 0006-291X
PURE UUID: 4e708a7f-1d94-4712-8934-1bcb3260afae
Catalogue record
Date deposited: 15 Aug 2012 10:22
Last modified: 15 Mar 2024 03:41
Export record
Altmetrics
Contributors
Author:
R.Z. Pino
Download statistics
Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.
View more statistics
