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Quantitative and kinetic characterization of nitric oxide and EDRF released from cultured endothelial cells

Kelm, Malte, Feelisch, Martin, Spahr, Rolf, Piper, Hans-Michael, Noack, Eike and Schrader, Jürgen (1988) Quantitative and kinetic characterization of nitric oxide and EDRF released from cultured endothelial cells Biochemical and Biophysical Research Communications, 154, (1), pp. 236-244. (doi:10.1016/0006-291X(88)90675-4). (PMID:3260776).

Record type: Article


Endothelial cells (EC) contribute to the control of local vascular diameter by formation of an endothelium derived relaxant factor (EDRF) (1). Whether nitric oxide (NO) is identical with (EDRF) or might represent only one species of several EDRFs has not been decided as yet (2-5). Therefore, we have directly compared in cultured EC the kinetics of NO formation determined in a photometric assay with the vasodilatory effect of EDRF and NO in a bioassay. Basal release of NO was 16, 4 pmol/min/ml packed EC column. After stimulation with bradykinin (BK) and ATP onset of endothelial NO release and maximal response preceded the EDRF-mediated relaxation. Concentrations of NO formed by stimulated EC were quantitatively sufficient to fully explain the smooth muscle relaxation determined in the bioassay. Our data provide convincing evidence that under basal, BK and ATP-stimulated conditions 1. endothelial cells release nitric oxide as free radical, 2. nitric oxide is solely responsible for the vasodilatory properties of EDRF.

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Published date: 15 July 1988
Organisations: Clinical & Experimental Sciences


Local EPrints ID: 337923
ISSN: 0006-291X
PURE UUID: ddd3e9f2-21d9-4af6-a5a6-b6a4e2837c69
ORCID for Martin Feelisch: ORCID iD

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Date deposited: 14 Jun 2012 11:37
Last modified: 18 Jul 2017 06:00

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Author: Malte Kelm
Author: Martin Feelisch ORCID iD
Author: Rolf Spahr
Author: Hans-Michael Piper
Author: Eike Noack
Author: Jürgen Schrader

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