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Quantitative and kinetic characterization of nitric oxide and EDRF released from cultured endothelial cells

Quantitative and kinetic characterization of nitric oxide and EDRF released from cultured endothelial cells
Quantitative and kinetic characterization of nitric oxide and EDRF released from cultured endothelial cells
Endothelial cells (EC) contribute to the control of local vascular diameter by formation of an endothelium derived relaxant factor (EDRF) (1). Whether nitric oxide (NO) is identical with (EDRF) or might represent only one species of several EDRFs has not been decided as yet (2-5). Therefore, we have directly compared in cultured EC the kinetics of NO formation determined in a photometric assay with the vasodilatory effect of EDRF and NO in a bioassay. Basal release of NO was 16, 4 pmol/min/ml packed EC column. After stimulation with bradykinin (BK) and ATP onset of endothelial NO release and maximal response preceded the EDRF-mediated relaxation. Concentrations of NO formed by stimulated EC were quantitatively sufficient to fully explain the smooth muscle relaxation determined in the bioassay. Our data provide convincing evidence that under basal, BK and ATP-stimulated conditions 1. endothelial cells release nitric oxide as free radical, 2. nitric oxide is solely responsible for the vasodilatory properties of EDRF.
0006-291X
236-244
Kelm, Malte
db2bb062-32d7-4b50-9f65-8ba89ffa5f42
Feelisch, Martin
8c1b9965-8614-4e85-b2c6-458a2e17eafd
Spahr, Rolf
f6351c5d-fa03-436d-a43c-f8e86ed658a6
Piper, Hans-Michael
db8c4c23-ab92-412c-875d-b866b02d7f78
Noack, Eike
dd3b6e9e-226e-4941-8d70-1c876d41fa50
Schrader, Jürgen
fb34ccfe-a295-411f-9ebb-f15b5ed4bad8
Kelm, Malte
db2bb062-32d7-4b50-9f65-8ba89ffa5f42
Feelisch, Martin
8c1b9965-8614-4e85-b2c6-458a2e17eafd
Spahr, Rolf
f6351c5d-fa03-436d-a43c-f8e86ed658a6
Piper, Hans-Michael
db8c4c23-ab92-412c-875d-b866b02d7f78
Noack, Eike
dd3b6e9e-226e-4941-8d70-1c876d41fa50
Schrader, Jürgen
fb34ccfe-a295-411f-9ebb-f15b5ed4bad8

Kelm, Malte, Feelisch, Martin, Spahr, Rolf, Piper, Hans-Michael, Noack, Eike and Schrader, Jürgen (1988) Quantitative and kinetic characterization of nitric oxide and EDRF released from cultured endothelial cells. Biochemical and Biophysical Research Communications, 154 (1), 236-244. (doi:10.1016/0006-291X(88)90675-4). (PMID:3260776)

Record type: Article

Abstract

Endothelial cells (EC) contribute to the control of local vascular diameter by formation of an endothelium derived relaxant factor (EDRF) (1). Whether nitric oxide (NO) is identical with (EDRF) or might represent only one species of several EDRFs has not been decided as yet (2-5). Therefore, we have directly compared in cultured EC the kinetics of NO formation determined in a photometric assay with the vasodilatory effect of EDRF and NO in a bioassay. Basal release of NO was 16, 4 pmol/min/ml packed EC column. After stimulation with bradykinin (BK) and ATP onset of endothelial NO release and maximal response preceded the EDRF-mediated relaxation. Concentrations of NO formed by stimulated EC were quantitatively sufficient to fully explain the smooth muscle relaxation determined in the bioassay. Our data provide convincing evidence that under basal, BK and ATP-stimulated conditions 1. endothelial cells release nitric oxide as free radical, 2. nitric oxide is solely responsible for the vasodilatory properties of EDRF.

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Published date: 15 July 1988
Organisations: Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 337923
URI: https://eprints.soton.ac.uk/id/eprint/337923
ISSN: 0006-291X
PURE UUID: ddd3e9f2-21d9-4af6-a5a6-b6a4e2837c69
ORCID for Martin Feelisch: ORCID iD orcid.org/0000-0003-2320-1158

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Date deposited: 14 Jun 2012 11:37
Last modified: 06 Jun 2018 12:29

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