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Nitric oxide (NO) formation from nitrovasodilators occurs independently of hemoglobin or non-heme iron

Nitric oxide (NO) formation from nitrovasodilators occurs independently of hemoglobin or non-heme iron
Nitric oxide (NO) formation from nitrovasodilators occurs independently of hemoglobin or non-heme iron
The aim of the present study was to exclude a potential role of hemoglobin in the formation of nitric oxide (NO) from several nitrovasodilators. NO was measured with a chemiluminescence technique after purging with argon from the aqueous solution. Nitric oxide generation occurred in the absence of hemoglobin or non-heme iron. Sodium nitroprusside and SIN-1 released NO spontaneously. Nitroglycerin produced NO only in the presence of those thiols which are effective co-stimulators of guanylate cyclase. All other thiols degraded nitroglycerin only into nitrite ions without formation of NO. Our results support the role of nitric oxide as terminal activator of guanylate cyclase stimulation by nitrovasodilators.
nitroglycerin, nitric oxide, thiols, nitrite, hemoglobin, guanylate cyclase, edrf (endothelium-derived relaxing factor)
0014-2999
465-469
Feelisch, Martin
8c1b9965-8614-4e85-b2c6-458a2e17eafd
Noack, Eike
dd3b6e9e-226e-4941-8d70-1c876d41fa50
Feelisch, Martin
8c1b9965-8614-4e85-b2c6-458a2e17eafd
Noack, Eike
dd3b6e9e-226e-4941-8d70-1c876d41fa50

Feelisch, Martin and Noack, Eike (1987) Nitric oxide (NO) formation from nitrovasodilators occurs independently of hemoglobin or non-heme iron. European Journal of Pharmacology, 142 (3), 465-469. (doi:10.1016/0014-2999(87)90090-2). (PMID:3123257)

Record type: Article

Abstract

The aim of the present study was to exclude a potential role of hemoglobin in the formation of nitric oxide (NO) from several nitrovasodilators. NO was measured with a chemiluminescence technique after purging with argon from the aqueous solution. Nitric oxide generation occurred in the absence of hemoglobin or non-heme iron. Sodium nitroprusside and SIN-1 released NO spontaneously. Nitroglycerin produced NO only in the presence of those thiols which are effective co-stimulators of guanylate cyclase. All other thiols degraded nitroglycerin only into nitrite ions without formation of NO. Our results support the role of nitric oxide as terminal activator of guanylate cyclase stimulation by nitrovasodilators.

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Published date: 27 October 1987
Keywords: nitroglycerin, nitric oxide, thiols, nitrite, hemoglobin, guanylate cyclase, edrf (endothelium-derived relaxing factor)
Organisations: Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 337927
URI: https://eprints.soton.ac.uk/id/eprint/337927
ISSN: 0014-2999
PURE UUID: b6038c7d-f798-40cb-b83b-b3ef065fc86c
ORCID for Martin Feelisch: ORCID iD orcid.org/0000-0003-2320-1158

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Date deposited: 14 Jun 2012 15:09
Last modified: 06 Jun 2018 12:29

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Author: Martin Feelisch ORCID iD
Author: Eike Noack

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