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Effectiveness of pressurised carbon dioxide for inactivation of Escherichia coli isolated from sewage sludge

Effectiveness of pressurised carbon dioxide for inactivation of Escherichia coli isolated from sewage sludge
Effectiveness of pressurised carbon dioxide for inactivation of Escherichia coli isolated from sewage sludge
This research explored the possible application of pressurised carbon dioxide (P CO2), a promising non-thermal sterilisation technique, for the treatment of sewage sludge (SS) before anaerobic digestion to inactivate pathogenic microorganisms. Escherichia coli was selected as the test organism and was isolated from SS and maintained in pure culture. The growth curve of the isolated strain was determined by measuring the optical density (OD) in liquid culture medium and relating this information to the spread plate count so that a culture of known cell density could be grown for optimisation experiments. Inactivation of E. coli was enhanced by increase in pressure (1,500, 2,000 and 2,800 kPa) and treatment time (from 0.75 to 24 h). A short exposure time at high pressure was sufficient to provide a degree of inactivation which could also be achieved by longer exposure at lower pressure. Complete inactivation (8 log10 reduction) was possible at all three pressures. scanning (SEM) and transmission (TEM) electron microscopy studies of E. coli treated with P CO2 revealed that the cell walls were ruptured, and the cytoplasm was unevenly distributed and had lost its density, indicating the possible leakage of intracellular substances.
anaerobic digestion, pathogens, pressurised carbon dioxide, sewage sludge
0273-1223
1759-1764
Mushtaq, Maryam
7046915f-8cea-4281-a3e3-c91e0eba6410
Banks, Charles J.
5c6c8c4b-5b25-4e37-9058-50fa8d2e926f
Heaven, Sonia
f25f74b6-97bd-4a18-b33b-a63084718571
Mushtaq, Maryam
7046915f-8cea-4281-a3e3-c91e0eba6410
Banks, Charles J.
5c6c8c4b-5b25-4e37-9058-50fa8d2e926f
Heaven, Sonia
f25f74b6-97bd-4a18-b33b-a63084718571

Mushtaq, Maryam, Banks, Charles J. and Heaven, Sonia (2012) Effectiveness of pressurised carbon dioxide for inactivation of Escherichia coli isolated from sewage sludge. Water Science & Technology, 65 (10), 1759-1764. (doi:10.2166/wst.2012.064). (PMID:22546789)

Record type: Article

Abstract

This research explored the possible application of pressurised carbon dioxide (P CO2), a promising non-thermal sterilisation technique, for the treatment of sewage sludge (SS) before anaerobic digestion to inactivate pathogenic microorganisms. Escherichia coli was selected as the test organism and was isolated from SS and maintained in pure culture. The growth curve of the isolated strain was determined by measuring the optical density (OD) in liquid culture medium and relating this information to the spread plate count so that a culture of known cell density could be grown for optimisation experiments. Inactivation of E. coli was enhanced by increase in pressure (1,500, 2,000 and 2,800 kPa) and treatment time (from 0.75 to 24 h). A short exposure time at high pressure was sufficient to provide a degree of inactivation which could also be achieved by longer exposure at lower pressure. Complete inactivation (8 log10 reduction) was possible at all three pressures. scanning (SEM) and transmission (TEM) electron microscopy studies of E. coli treated with P CO2 revealed that the cell walls were ruptured, and the cytoplasm was unevenly distributed and had lost its density, indicating the possible leakage of intracellular substances.

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More information

Published date: 2012
Keywords: anaerobic digestion, pathogens, pressurised carbon dioxide, sewage sludge
Organisations: Centre for Environmental Science

Identifiers

Local EPrints ID: 337986
URI: http://eprints.soton.ac.uk/id/eprint/337986
ISSN: 0273-1223
PURE UUID: b816f7e5-6612-480f-9451-83d6eee12882
ORCID for Sonia Heaven: ORCID iD orcid.org/0000-0001-7798-4683

Catalogue record

Date deposited: 09 May 2012 12:15
Last modified: 17 Mar 2020 01:25

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