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DNA from soil mirrors plant taxonomic and growth form diversity

Yoccoz, N.G., Brathen, K.A. and Geilly, L. et al. (2012) DNA from soil mirrors plant taxonomic and growth form diversity Molecular Ecology, 21, (15), pp. 3647-3655. (doi:10.1111/j.1365-294X.2012.05545.x).

Record type: Article

Abstract

Ecosystems across the globe are threatened by climate change and human activities. New rapid survey approaches for monitoring biodiversity would greatly advance assessment and understanding of these threats. Taking advantage of next-generation DNA sequencing, we tested an approach we call metabarcoding: high-throughput and simultaneous taxa identification based on a very short (usually <100 base pairs) but informative DNA fragment. Short DNA fragments allow the use of degraded DNA from environmental samples. All analyses included amplification using plant-specific versatile primers, sequencing and estimation of taxonomic diversity. We tested in three steps whether degraded DNA from dead material in soil has the potential of efficiently assessing biodiversity in different biomes. First, soil DNA from eight boreal plant communities located in two different vegetation types (meadow and heath) was amplified. Plant diversity detected from boreal soil was highly consistent with plant taxonomic and growth form diversity estimated from conventional above-ground surveys. Second, we assessed DNA persistence using samples from formerly cultivated soils in temperate environments. We found that the number of crop DNA sequences retrieved strongly varied with years since last cultivation, and crop sequences were absent from nearby, uncultivated plots. Third, we assessed the universal applicability of DNA metabarcoding using soil samples from tropical environments: a large proportion of species and families from the study site were efficiently recovered. The results open unprecedented opportunities for large-scale DNA-based biodiversity studies across a range of taxonomic groups using standardized metabarcoding approaches

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Published date: 16 April 2012
Keywords: biodiversity assessment, DNA metabarcoding, environmental sequencing, functional diversity, plant diversity
Organisations: Palaeoenvironment Laboratory (PLUS)

Identifiers

Local EPrints ID: 338783
URI: http://eprints.soton.ac.uk/id/eprint/338783
ISSN: 0962-1083
PURE UUID: e785ca30-ba59-49bf-905a-4074a6692372
ORCID for M.E. Edwards: ORCID iD orcid.org/0000-0002-3490-6682

Catalogue record

Date deposited: 17 May 2012 12:56
Last modified: 18 Jul 2017 05:57

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Contributors

Author: N.G. Yoccoz
Author: K.A. Brathen
Author: L. Geilly
Author: J. Haile
Author: M.E. Edwards ORCID iD
Author: T. Goslar
Author: H. von Stedingk
Author: E. Coissac
Author: F. Pompanon
Author: J.H. Sonstebo
Author: C. Miquel
Author: A. Valentini
Author: F. De Bello
Author: J. Chave
Author: W. Thuiller
Author: P. Wincker
Author: C. Cruaud
Author: F. Gavory
Author: M. Rasmussen
Author: M.T.P Gilbert
Author: L. Orlando
Author: C. Brochmann
Author: E. Willerslev
Author: P. Taberlet

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