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Surfactant protein D (SP-D) alters cellular uptake of particles and nanoparticles

Kendall, M., Ding, P, Mackay, Rose-Marie, Madsen, Jens, Deb, R., Mackenzie, Z, Kendall, K. and Clark, H.W. (2013) Surfactant protein D (SP-D) alters cellular uptake of particles and nanoparticles Nanotoxicology, 7, (5), pp. 963-973. (doi:10.3109/17435390.2012.689880). (PMID:22551051).

Record type: Article


Surfactant protein D (SP-D) is primarily expressed in the lungs and modulates pro- and anti- inflammatory processes to toxic challenge, maintaining lung homeostasis. We investigated the interaction between NPs and SP-D and subsequent uptake by cells involved in lung immunity. Dynamic light scattering (DLS) and scanning electron microscopy (SEM) measuredNP aggregation, particle size and charge in native human SP-D (NhSP-D) and recombinant fragment SP-D (rfhSP-D). Immunohistochemical analysis of A549 epithelial cells investigated the co-localization of NPs and rfhSP-D. NP uptake by alveolar macrophages (AMs) and lung dendritic cells (LDCs) from C57BL/6 and SP-D knock-out mice were compared.SP-D aggregated NPs, especially following the addition of calcium. rfhSP-D enhanced the co-localisation of NPs to epithelial A459 cells in vitro. AMs and LDCs showed decreased uptake of NPs in SP-D deficient mice compared to wild-type mice. These data confirmed an interaction between SP-D and NPs, and subsequent enhanced NP uptake.

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Published date: August 2013
Organisations: Clinical & Experimental Sciences


Local EPrints ID: 338828
ISSN: 1743-5390
PURE UUID: e064cead-43ff-452d-b27c-3e4e31c70e4c
ORCID for Rose-Marie Mackay: ORCID iD
ORCID for Jens Madsen: ORCID iD

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Date deposited: 17 May 2012 14:30
Last modified: 18 Jul 2017 05:57

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Author: M. Kendall
Author: P Ding
Author: Rose-Marie Mackay ORCID iD
Author: Jens Madsen ORCID iD
Author: R. Deb
Author: Z Mackenzie
Author: K. Kendall
Author: H.W. Clark

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