Kendall, M., Ding, P, Mackay, Rose-Marie, Madsen, Jens, Deb, R., Mackenzie, Z, Kendall, K. and Clark, H.W.
Surfactant protein D (SP-D) alters cellular uptake of particles and nanoparticles
Nanotoxicology, 7, (5), . (doi:10.3109/17435390.2012.689880). (PMID:22551051).
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Surfactant protein D (SP-D) is primarily expressed in the lungs and modulates pro- and anti- inflammatory processes to toxic challenge, maintaining lung homeostasis. We investigated the interaction between NPs and SP-D and subsequent uptake by cells involved in lung immunity. Dynamic light scattering (DLS) and scanning electron microscopy (SEM) measuredNP aggregation, particle size and charge in native human SP-D (NhSP-D) and recombinant fragment SP-D (rfhSP-D). Immunohistochemical analysis of A549 epithelial cells investigated the co-localization of NPs and rfhSP-D. NP uptake by alveolar macrophages (AMs) and lung dendritic cells (LDCs) from C57BL/6 and SP-D knock-out mice were compared.SP-D aggregated NPs, especially following the addition of calcium. rfhSP-D enhanced the co-localisation of NPs to epithelial A459 cells in vitro. AMs and LDCs showed decreased uptake of NPs in SP-D deficient mice compared to wild-type mice. These data confirmed an interaction between SP-D and NPs, and subsequent enhanced NP uptake.
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