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Developing methacrylate-based copolymers as an artificial Bruch's membrane substitute

Developing methacrylate-based copolymers as an artificial Bruch's membrane substitute
Developing methacrylate-based copolymers as an artificial Bruch's membrane substitute
Age-related macular degeneration (AMD) is the most common cause of blindness in the developed world. There is currently no treatment for the cellular loss, which is characteristic of AMD. Transplantation of retinal pigment epithelium (RPE) cells represents a potential therapy. Because of AMD-related pathology in the native support, Bruch's membrane, transplanted RPE cells require a scaffold to reside on. We present here the development of an electrospun fibrous scaffold derived from methyl methacrylate and poly(ethylene glycol) (PEG) methacrylate for novel application as an RPE scaffold. Scaffolds were chemically modified to improve cell adhesion by functionalization not previously reported for this type of copolymer system. A human RPE cell line was used to investigate cell-scaffold interactions for up to two weeks in vitro. Scanning electron microscopy was used to characterize the fibrous scaffolds and confirm cell attachment. By day 15, cell area was significantly (p < 0.001) enhanced on scaffolds with chemical modification of the PEG chain terminus. In addition, significantly, less-apoptotic cell death was demonstrable on these modified surfaces. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.
copolymer, ophthalmology, retina, RGD peptide, scaffold, polymethylmethacrylate
1549-3296
Treharne, Andrew John
a158bb09-9d41-4b3a-ae02-ccbd76dd9417
Thomson, Heather A.J.
21bbe4d3-6593-42f6-91c8-5d856f5da378
Grossel, Martin C.
403bf3ff-6364-44e9-ab46-52d84c6f0d56
Lotery, Andrew J.
5ecc2d2d-d0b4-468f-ad2c-df7156f8e514
Treharne, Andrew John
a158bb09-9d41-4b3a-ae02-ccbd76dd9417
Thomson, Heather A.J.
21bbe4d3-6593-42f6-91c8-5d856f5da378
Grossel, Martin C.
403bf3ff-6364-44e9-ab46-52d84c6f0d56
Lotery, Andrew J.
5ecc2d2d-d0b4-468f-ad2c-df7156f8e514

Treharne, Andrew John, Thomson, Heather A.J., Grossel, Martin C. and Lotery, Andrew J. (2012) Developing methacrylate-based copolymers as an artificial Bruch's membrane substitute. Journal of Biomedical Materials Research Part A. (doi:10.1002/jbm.a.34178). (PMID:22528296)

Record type: Article

Abstract

Age-related macular degeneration (AMD) is the most common cause of blindness in the developed world. There is currently no treatment for the cellular loss, which is characteristic of AMD. Transplantation of retinal pigment epithelium (RPE) cells represents a potential therapy. Because of AMD-related pathology in the native support, Bruch's membrane, transplanted RPE cells require a scaffold to reside on. We present here the development of an electrospun fibrous scaffold derived from methyl methacrylate and poly(ethylene glycol) (PEG) methacrylate for novel application as an RPE scaffold. Scaffolds were chemically modified to improve cell adhesion by functionalization not previously reported for this type of copolymer system. A human RPE cell line was used to investigate cell-scaffold interactions for up to two weeks in vitro. Scanning electron microscopy was used to characterize the fibrous scaffolds and confirm cell attachment. By day 15, cell area was significantly (p < 0.001) enhanced on scaffolds with chemical modification of the PEG chain terminus. In addition, significantly, less-apoptotic cell death was demonstrable on these modified surfaces. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.

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More information

e-pub ahead of print date: 24 April 2012
Published date: September 2012
Keywords: copolymer, ophthalmology, retina, RGD peptide, scaffold, polymethylmethacrylate
Organisations: Chemistry, Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 338835
URI: http://eprints.soton.ac.uk/id/eprint/338835
ISSN: 1549-3296
PURE UUID: 3a4117c9-3a28-43f8-bccd-987599a9253d
ORCID for Martin C. Grossel: ORCID iD orcid.org/0000-0001-7469-6854
ORCID for Andrew J. Lotery: ORCID iD orcid.org/0000-0001-5541-4305

Catalogue record

Date deposited: 17 May 2012 15:13
Last modified: 15 Sep 2021 01:47

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Contributors

Author: Andrew John Treharne
Author: Heather A.J. Thomson

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