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Temperature-dependent global gene expression in the Antarctic archaeon Methanococcoides burtonii

Record type: Article

Methanococcoides burtonii is a member of the Archaea that was isolated from Ace Lake in Antarctica and is a valuable model for studying cold adaptation. Low temperature transcriptional regulation of global gene expression, and the arrangement of transcriptional units in cold-adapted archaea has not been studied. We developed a microarray for determining which genes are expressed in operons, and which are differentially expressed at low (4°C) or high (23°C) temperature. Approximately 55% of genes were found to be arranged in operons that range in length from 2 to 23 genes, and mRNA abundance tended to increase with operon length. Analysing microarray data previously obtained by others for Halobacterium salinarum revealed a similar correlation between operon length and mRNA abundance, suggesting that operons may play a similar role more broadly in the Archaea. More than 500 genes were differentially expressed at levels up to ? 24-fold. A notable feature was the upregulation of genes involved in maintaining RNA in a state suitable for translation in the cold. Comparison between microarray experiments and results previously obtained using proteomics indicates that transcriptional regulation (rather than translation) is primarily responsible for controlling gene expression in M. burtonii. In addition, certain genes (e.g. involved in ribosome structure and methanogenesis) appear to be regulated post-transcriptionally. This is one of few experimental studies describing the genome-wide distribution and regulation of operons in archaea.

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Citation

Campanaro, S., Williams, T.J., Burg, D.W., De Francisci, D., Treu, L., Lauro, F.M. and Cavicchioli, R. (2011) Temperature-dependent global gene expression in the Antarctic archaeon Methanococcoides burtonii Environmental Microbiology, 13, (8), pp. 2018-2038. (doi:10.1111/j.1462-2920.2010.02367.x). (PMID:21059163).

More information

e-pub ahead of print date: 8 November 2010
Published date: August 2011
Organisations: Molecular and Cellular

Identifiers

Local EPrints ID: 338907
URI: http://eprints.soton.ac.uk/id/eprint/338907
ISSN: 1462-2920
PURE UUID: e5bc7d72-c035-4d83-881e-8b52b67d0fd6

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Date deposited: 18 May 2012 13:35
Last modified: 18 Jul 2017 05:56

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Contributors

Author: S. Campanaro
Author: T.J. Williams
Author: D.W. Burg
Author: D. De Francisci
Author: L. Treu
Author: F.M. Lauro
Author: R. Cavicchioli

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