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Docosahexaenoic acid inhibits the adhesion of flowing neutrophils to cytokine stimulated human umbilical vein endothelial cells

Yates, Clara M., Tull, Samantha P., Madden, Jackie, Calder, Philip C., Grimble, Robert F., Nash, Gerard B. and Rainger, G. Ed (2011) Docosahexaenoic acid inhibits the adhesion of flowing neutrophils to cytokine stimulated human umbilical vein endothelial cells Journal of Nutrition, 141, (7), pp. 1331-1334. (doi:10.3945/jn.111.139287). (PMID:21613456).

Record type: Article


The (n-3) PUFA, DHA, is widely thought to posses the ability to modulate the inflammatory response. However, its modes of interaction with inflammatory cells are poorly understood. In particular, there are limited data on the interactions of DHA with vascular endothelium, the cells that regulate the traffic of leukocytes from the blood into inflamed tissue. Using human umbilical vein endothelial cells (EC) cultured in a flow-based adhesion assay and activated with TNF?, we tested whether supplementing human umbilical vein EC with physiologically achievable concentrations of DHA would inhibit the recruitment of flowing neutrophils. DHA caused a dose-dependent reduction in neutrophil recruitment to the EC surface, although cells that became adherent were activated and could migrate across the human umbilical vein EC monolayer normally. Using EPA as an alternative supplement had no effect on the levels of neutrophil adhesion in this assay. Analysis of adhesion receptor expression by qPCR demonstrated that DHA did not alter the transcriptional activity of human umbilical vein EC. However, DHA did significantly reduce E-selectin expression at the human umbilical vein EC surface without altering the total cellular pool of this adhesion receptor. Thus, we have identified a novel mechanism by which DHA alters the trafficking of leukocytes during inflammation and demonstrate that this involves disruption of intracellular transport mechanisms used to present adhesion molecules on the surface of cytokine-stimulated EC.

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Published date: 1 July 2011
Organisations: Human Development & Health


Local EPrints ID: 339036
ISSN: 0022-3166
PURE UUID: f37e619a-f163-415c-a3b0-f9842f76bd2d

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Date deposited: 22 May 2012 10:01
Last modified: 18 Jul 2017 05:55

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Author: Clara M. Yates
Author: Samantha P. Tull
Author: Jackie Madden
Author: Robert F. Grimble
Author: Gerard B. Nash
Author: G. Ed Rainger

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