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Genotyping markers used for multi locus VNTR analysis with ompA (MLVA-ompA) and multi sequence typing (MST) retain stability in Chlamydia trachomatis

Labiran, C., Clarke, Ian N., Cutcliffe, Lesley T., Wang, Yibing, Skilton, Rachel J., Persson, Kenneth, Bjartling, Carina, Herrmann, Björn, Christerson, Linus and Marsh, Peter (2012) Genotyping markers used for multi locus VNTR analysis with ompA (MLVA-ompA) and multi sequence typing (MST) retain stability in Chlamydia trachomatis Frontiers in Cellular and Infection Microbiology, 2, (68) (doi:10.3389/fcimb.2012.00068).

Record type: Article

Abstract

We aimed to evaluate the stability of the Chlamydia trachomatis multi locus VNTR analysis (MLVA-ompA) and multi sequence typing (MST) systems through multiple passages in tissue culture. Firstly, we analyzed the stability of these markers through adaptation of C. trachomatis to tissue culture and secondly, we examined the stability of a four-locus MLVA-ompA and a five-locus MST system after multiple passages in tissue culture. Marker sequences were monitored through successive chlamydial developmental cycles to evaluate the stability of the individual DNA markers through many bacterial divisions and this, in turn, informed us of the usefulness of using such typing systems for short and long-term molecular epidemiology. Southampton genitourinary medicine (GUM) clinic isolates from endocervical swabs collected from C. trachomatis positive women were passaged through tissue culture. MLVA-ompA typing was applied to primary swab samples and to the same samples after C. trachomatis had been passaged through cell culture (eight passages). Sequence data from time-zero and passage-eight isolates were aligned with reference sequences to determine the stability of the markers. The Swedish new variant (nvCT) underwent 72 passages in cell culture and the markers of the two schemes were similarly analyzed. Analysis of genetic markers of the MLVA-ompA typing system before and after the isolates were introduced to tissue culture showed no change in the dominant sequence. The nvCT that had been passaged 72 times over the duration of a year also showed no variation in the dominant sequence for both the genotyping schemes. MLVA-ompA and MST markers are stable upon adaptation of C. trachomatis to tissue culture following isolation of strains from primary endocervical swab samples. These markers remain stable throughout multiple rounds of cell-division in tissue culture, concomitant with the incubation period and appearance of symptoms normally associated with host-infection. Both genotyping schemes are, therefore, suitable for epidemiology of C. trachomatis.

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Published date: 17 May 2012
Organisations: Clinical & Experimental Sciences

Identifiers

Local EPrints ID: 339149
URI: http://eprints.soton.ac.uk/id/eprint/339149
PURE UUID: 13ef2bb3-2489-4328-a7e6-2d37e23b964e
ORCID for Ian N. Clarke: ORCID iD orcid.org/0000-0002-4938-1620

Catalogue record

Date deposited: 24 May 2012 13:06
Last modified: 18 Jul 2017 05:54

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Contributors

Author: C. Labiran
Author: Ian N. Clarke ORCID iD
Author: Lesley T. Cutcliffe
Author: Yibing Wang
Author: Rachel J. Skilton
Author: Kenneth Persson
Author: Carina Bjartling
Author: Björn Herrmann
Author: Linus Christerson
Author: Peter Marsh

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