The University of Southampton
University of Southampton Institutional Repository

Death receptor 3 is essential for generating optimal protective CD41 T-cell immunity against Salmonella

Buchan, Sarah L., Taraban, Vadim Y., Slebioda, Tomasz J., James, Sonya, Cunningham, Adam F. and Al-Shamkhani, Aymen (2012) Death receptor 3 is essential for generating optimal protective CD41 T-cell immunity against Salmonella European Journal of Immunology, 42, (3), pp. 580-588. (doi:10.1002/eji.201041950). (PMID:22259035).

Record type: Article


The TNF receptor superfamily member death receptor 3 (DR3) exacerbates Th2- and Th17-cell-mediated inflammatory and autoimmune conditions, yet no role in host defence has been reported. Here, we examined the role of DR3 during infection with Salmonella enterica serovar Typhimurium. Infection resulted in protracted expression of the DR3 ligand TL1A but not the related TNF superfamily proteins OX40L or CD30L. TL1A expression was localized to splenic F4/80+ macrophages where S. enterica Typhimurium replicates, and temporally coincided with the onset of CD4+-cell expansion. To address the relevance of the TL1A-DR3 interaction, we examined immune responses to S. enterica Typhimurium in mice lacking DR3. Infected DR3-/- mice harboured reduced numbers of antigen-experienced and proliferating CD4+ T cells compared with WT mice. Furthermore, the frequency of IFN-?+ CD4+ T cells in DR3-/- mice was lower throughout the time of bacterial clearance. Importantly, bacterial clearance, which is dependent on Th1 cells, was also impaired in DR3-/- mice. This defect was intrinsic to CD4+ T cells as evidenced by an increase in bacterial burden in RAG2-deficient mice receiving DR3-/- CD4+ T cells compared with WT CD4+-cell recipients. These data establish for the first time a role for DR3 in a host defence responce.

Full text not available from this repository.

More information

Accepted/In Press date: 16 November 2011
e-pub ahead of print date: 19 January 2012
Published date: March 2012
Keywords: costimulation, DR3, Th1 cells, TNF receptor superfamily, TNFRSF25
Organisations: Cancer Sciences


Local EPrints ID: 339281
ISSN: 0014-2980
PURE UUID: e3c9b2c2-223d-41ed-9c7c-2756139b1d6e

Catalogue record

Date deposited: 25 May 2012 11:40
Last modified: 18 Jul 2017 05:53

Export record



Author: Sarah L. Buchan
Author: Vadim Y. Taraban
Author: Tomasz J. Slebioda
Author: Sonya James
Author: Adam F. Cunningham

University divisions

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton:

ePrints Soton supports OAI 2.0 with a base URL of

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.