Cell migration and invasion assays
Cell migration and invasion assays
A number of in vitro assays have been developed to study tumor cell motility. Historically, assays have been mainly monocellular, where carcinoma cells are studied in isolation. Scratch assays can be used to study the collective and directional movement of populations of cells, whereas two chamber assays lend themselves to the analysis of chemotactic/haptotactic migration and cell invasion. However, an inherent disadvantage of these assays is that they grossly oversimplify the complex process of invasion, lacking the tumor structural architecture and stromal components. Organotypic assays, where tumor cells are grown at an air/liquid interface on gels populated with stromal cells, are a more physiologically relevant method for studying 3-dimensional tumor invasion.
invasion, migration, scratch assay, transwell, matrix, organotypic culture
333-343
Moutasim, Karwan A.
af7dd711-f6df-44f7-8c57-052bf15303af
Nystrom, Maria L.
d3ca00b3-f18c-46b5-9e8c-08ef34987015
Thomas, Gareth J.
2ff54aa9-a766-416b-91ee-cf1c5be74106
2011
Moutasim, Karwan A.
af7dd711-f6df-44f7-8c57-052bf15303af
Nystrom, Maria L.
d3ca00b3-f18c-46b5-9e8c-08ef34987015
Thomas, Gareth J.
2ff54aa9-a766-416b-91ee-cf1c5be74106
Moutasim, Karwan A., Nystrom, Maria L. and Thomas, Gareth J.
(2011)
Cell migration and invasion assays.
[in special issue: Cancer Cell Culture]
Methods in Molecular Biology, 731, .
(doi:10.1007/978-1-61779-080-5_27).
(PMID:21516419)
Abstract
A number of in vitro assays have been developed to study tumor cell motility. Historically, assays have been mainly monocellular, where carcinoma cells are studied in isolation. Scratch assays can be used to study the collective and directional movement of populations of cells, whereas two chamber assays lend themselves to the analysis of chemotactic/haptotactic migration and cell invasion. However, an inherent disadvantage of these assays is that they grossly oversimplify the complex process of invasion, lacking the tumor structural architecture and stromal components. Organotypic assays, where tumor cells are grown at an air/liquid interface on gels populated with stromal cells, are a more physiologically relevant method for studying 3-dimensional tumor invasion.
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Published date: 2011
Keywords:
invasion, migration, scratch assay, transwell, matrix, organotypic culture
Organisations:
Cancer Sciences
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Local EPrints ID: 339691
URI: http://eprints.soton.ac.uk/id/eprint/339691
PURE UUID: 67d79286-36d1-4c87-8515-fb1054ead673
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Date deposited: 29 May 2012 11:11
Last modified: 14 Mar 2024 11:14
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Author:
Maria L. Nystrom
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