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Cell migration and invasion assays

Moutasim, Karwan A., Nystrom, Maria L. and Thomas, Gareth J. (2011) Cell migration and invasion assays [in special issue: Cancer Cell Culture] Methods in Molecular Biology, 731, pp. 333-343. (doi:10.1007/978-1-61779-080-5_27). (PMID:21516419).

Record type: Article

Abstract

A number of in vitro assays have been developed to study tumor cell motility. Historically, assays have been mainly monocellular, where carcinoma cells are studied in isolation. Scratch assays can be used to study the collective and directional movement of populations of cells, whereas two chamber assays lend themselves to the analysis of chemotactic/haptotactic migration and cell invasion. However, an inherent disadvantage of these assays is that they grossly oversimplify the complex process of invasion, lacking the tumor structural architecture and stromal components. Organotypic assays, where tumor cells are grown at an air/liquid interface on gels populated with stromal cells, are a more physiologically relevant method for studying 3-dimensional tumor invasion.

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More information

Published date: 2011
Keywords: invasion, migration, scratch assay, transwell, matrix, organotypic culture
Organisations: Cancer Sciences

Identifiers

Local EPrints ID: 339691
URI: http://eprints.soton.ac.uk/id/eprint/339691
PURE UUID: 67d79286-36d1-4c87-8515-fb1054ead673

Catalogue record

Date deposited: 29 May 2012 11:11
Last modified: 18 Jul 2017 05:52

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Contributors

Author: Maria L. Nystrom

University divisions

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