Lipid layers on polyelectrolyte multilayer supports
Martin
Fischlechner
,
Markus
Zaulig
,
Stefan
Meyer
,
Irina
Estrela-Lopis
,
Luis
Cuéllar
,
Joseba
Irigoyen
,
Paula
Pescador
,
Milan
Brumen
,
Paul
Messner
,
Sergio
Moya
and
Edwin
Donath
Soft Matter, 2008,4, 2245-2258
DOI:
10.1039/B805754K
Received
07 Apr 2008,
Accepted
30 Jun 2008
First published on the web
18 Aug 2008
The mechanism of formation of supported lipid layers from phosphatidylcholine and phosphatidylserinevesicles in solution on polyelectrolyte multilayers was studied by a variety of experimental techniques. The interaction of zwitterionic and acidic lipidvesicles, as well as their mixtures, with polyelectrolyte supports was followed in real time by micro-gravimetry. The fabricated lipid–polyelectrolyte composite structures on top of multilayer coated colloidal particles were characterized by flow cytometry and imaging techniques. Lipid diffusion over the macroscopic scale was quantified by fluorescence recovery after photobleaching, and the diffusion was related to layer connectivity. The phospholipid–polyelectrolyte binding mechanism was investigated by infrared spectroscopy. A strong interaction of polyelectrolyte primary amino groups with phosphate and carboxylgroups of the phospholipids, leading to dehydration, was observed. Long-range electrostatic attraction was proven to be essential for vesicle spreading and rupture. Fusion of lipid patches into a homogeneous bilayer required lateral mobility of the lipids on the polyelectrolyte support. The binding of amino groups to the phosphate group of the zwitterionic lipids was too weak to induce vesicle spreading, but sufficient for strong adsorption. Only the mixture of phosphatidylcholine and phosphatidylserine resulted in the spontaneous formation of bilayers on polyelectrolyte multilayers. The adsorption of phospholipids onto multilayers displaying quarternary ammoniumpolymers produced a novel 3D lipidpolyelectrolyte structure on colloidal particles.
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