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Characterization of lipid bilayers adsorbed on spherical LbL-support

Characterization of lipid bilayers adsorbed on spherical LbL-support
Characterization of lipid bilayers adsorbed on spherical LbL-support
Structural and dynamic properties of membranes composed of phosphatidylcholine (PC) and phosphatidylserine (PS) on layer-by-layer (LbL) polyelectrolyte coated particles were investigated using solid-state nuclear magnetic resonance (NMR) and fluorescence methods. These spherically supported membranes showed structural, dynamic, and elastic properties similar to free-standing membranes as proved by 31P and 2H NMR. Small differences between behaviour of PC and PS on LbL support due to interaction with the polyelectrolyte were observed. Fluorescence lifetime imaging microscopy (FLIM) using 7-nitro-2-1,3-benzoxadiazol (NBD) labeled PC and PS showed a stronger impact of the outermost polyelectrolyte (PAH) on the fluorescence lifetimes of NBD-PS compared to NBD-PC. Although small defects in nm range allowing passage of Mn2+ to both layers of the membrane coat were present, a rather homogeneous coating observed by fluorescence microscopy, complete fluorescence recovery after photobleaching, and NMR results reveal that somewhat continuous lipid bilayers were formed around the LbL particles



1744-683X
3331-339
Bunge, Andreas
be35faba-b1b1-4720-af01-46a2b6b9d0ee
Fischlechner, Martin
b3930129-0775-4c05-81c7-475934df97ee
Loew, Martin
1c967a91-f1ad-43bc-a42d-eccbc2eb29ad
Arbuzova, Anna
1ecb997e-4fc2-4532-83b0-a626ae0f6602
Herrmann, Andreas
99fd177c-64d3-4325-b00b-eb7aaee35b68
Huster, Daniel
272f7795-4189-43e8-b1a6-b0e503dfeaab
Bunge, Andreas
be35faba-b1b1-4720-af01-46a2b6b9d0ee
Fischlechner, Martin
b3930129-0775-4c05-81c7-475934df97ee
Loew, Martin
1c967a91-f1ad-43bc-a42d-eccbc2eb29ad
Arbuzova, Anna
1ecb997e-4fc2-4532-83b0-a626ae0f6602
Herrmann, Andreas
99fd177c-64d3-4325-b00b-eb7aaee35b68
Huster, Daniel
272f7795-4189-43e8-b1a6-b0e503dfeaab

Bunge, Andreas, Fischlechner, Martin, Loew, Martin, Arbuzova, Anna, Herrmann, Andreas and Huster, Daniel (2009) Characterization of lipid bilayers adsorbed on spherical LbL-support. Soft Matter, 5 (17), 3331-339. (doi:10.1039/b902264c).

Record type: Article

Abstract

Structural and dynamic properties of membranes composed of phosphatidylcholine (PC) and phosphatidylserine (PS) on layer-by-layer (LbL) polyelectrolyte coated particles were investigated using solid-state nuclear magnetic resonance (NMR) and fluorescence methods. These spherically supported membranes showed structural, dynamic, and elastic properties similar to free-standing membranes as proved by 31P and 2H NMR. Small differences between behaviour of PC and PS on LbL support due to interaction with the polyelectrolyte were observed. Fluorescence lifetime imaging microscopy (FLIM) using 7-nitro-2-1,3-benzoxadiazol (NBD) labeled PC and PS showed a stronger impact of the outermost polyelectrolyte (PAH) on the fluorescence lifetimes of NBD-PS compared to NBD-PC. Although small defects in nm range allowing passage of Mn2+ to both layers of the membrane coat were present, a rather homogeneous coating observed by fluorescence microscopy, complete fluorescence recovery after photobleaching, and NMR results reveal that somewhat continuous lipid bilayers were formed around the LbL particles



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Published date: 25 June 2009
Organisations: Chemistry

Identifiers

Local EPrints ID: 340195
URI: https://eprints.soton.ac.uk/id/eprint/340195
ISSN: 1744-683X
PURE UUID: 6e001d30-b7a8-4609-b0da-42bec3fe4b60

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Date deposited: 14 Jun 2012 14:32
Last modified: 18 Jul 2017 05:47

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