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New detection modality for label-free quantification of DNA in biological samples via superparamagnetic bead aggregation

New detection modality for label-free quantification of DNA in biological samples via superparamagnetic bead aggregation
New detection modality for label-free quantification of DNA in biological samples via superparamagnetic bead aggregation
Combining DNA and superparamagnetic beads in a rotating magnetic field produces multiparticle aggregates that are visually striking, enabling label-free optical detection and quantification of DNA at levels in the picogram per microliter range. DNA in biological samples can be quantified directly by simple analysis of optical images of microfluidic wells placed on a magnetic stirrer without prior DNA purification. Aggregation results from DNA/bead interactions driven either by the presence of a chaotrope (a nonspecific trigger for aggregation) or by hybridization with oligonucleotides on functionalized beads (sequence-specific). This paper demonstrates quantification of DNA with sensitivity comparable to that of the best currently available fluorometric assays. The robustness and sensitivity of the method enable a wide range of applications, illustrated here by counting eukaryotic cells. Using widely available and inexpensive benchtop hardware, the approach provides a highly accessible low-tech microscale alternative to more expensive DNA detection and cell counting techniques
0002-7863
5689-5696
Leslie, Daniel C.
cfba1079-7234-4c99-a459-3501f1ce5df5
Li, Jingyi
e4d0d0b6-98aa-48b2-bbfe-d63f4198c8e2
Strachan, Briony C.
e3a4a809-fef7-47e7-9e1e-e1ec6fb79950
Begley, Matthew R.
9f4e52bc-507a-4ef6-910f-bd11d25c2209
Finkler, David
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Bazydlo, Lindsay A. L.
a1fffc12-6592-42f7-a67e-f5489b1a420a
Barker, N. Scott
f3257bd2-e3dc-465d-839e-113cb3df7d9c
Haverstick, Doris M.
35bdafaa-50c1-407c-abf8-d469dfdf52d2
Utz, Marcel
c84ed64c-9e89-4051-af39-d401e423891b
Landers, James P.
f85edaa2-5c24-4f2d-be3d-c84e631833ad
Leslie, Daniel C.
cfba1079-7234-4c99-a459-3501f1ce5df5
Li, Jingyi
e4d0d0b6-98aa-48b2-bbfe-d63f4198c8e2
Strachan, Briony C.
e3a4a809-fef7-47e7-9e1e-e1ec6fb79950
Begley, Matthew R.
9f4e52bc-507a-4ef6-910f-bd11d25c2209
Finkler, David
083bcf2d-0f2e-4c07-b29d-49c538aa8ede
Bazydlo, Lindsay A. L.
a1fffc12-6592-42f7-a67e-f5489b1a420a
Barker, N. Scott
f3257bd2-e3dc-465d-839e-113cb3df7d9c
Haverstick, Doris M.
35bdafaa-50c1-407c-abf8-d469dfdf52d2
Utz, Marcel
c84ed64c-9e89-4051-af39-d401e423891b
Landers, James P.
f85edaa2-5c24-4f2d-be3d-c84e631833ad

Leslie, Daniel C., Li, Jingyi, Strachan, Briony C., Begley, Matthew R., Finkler, David, Bazydlo, Lindsay A. L., Barker, N. Scott, Haverstick, Doris M., Utz, Marcel and Landers, James P. (2012) New detection modality for label-free quantification of DNA in biological samples via superparamagnetic bead aggregation. Journal of the American Chemical Society, 134 (12), 5689-5696. (doi:10.1021/ja300839n).

Record type: Article

Abstract

Combining DNA and superparamagnetic beads in a rotating magnetic field produces multiparticle aggregates that are visually striking, enabling label-free optical detection and quantification of DNA at levels in the picogram per microliter range. DNA in biological samples can be quantified directly by simple analysis of optical images of microfluidic wells placed on a magnetic stirrer without prior DNA purification. Aggregation results from DNA/bead interactions driven either by the presence of a chaotrope (a nonspecific trigger for aggregation) or by hybridization with oligonucleotides on functionalized beads (sequence-specific). This paper demonstrates quantification of DNA with sensitivity comparable to that of the best currently available fluorometric assays. The robustness and sensitivity of the method enable a wide range of applications, illustrated here by counting eukaryotic cells. Using widely available and inexpensive benchtop hardware, the approach provides a highly accessible low-tech microscale alternative to more expensive DNA detection and cell counting techniques

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Published date: 16 March 2012
Organisations: Chemistry

Identifiers

Local EPrints ID: 340204
URI: http://eprints.soton.ac.uk/id/eprint/340204
ISSN: 0002-7863
PURE UUID: 90265dff-029a-4836-ac05-4f81eb25e273
ORCID for Marcel Utz: ORCID iD orcid.org/0000-0003-2274-9672

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Date deposited: 15 Jun 2012 08:11
Last modified: 17 Dec 2019 01:37

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Contributors

Author: Daniel C. Leslie
Author: Jingyi Li
Author: Briony C. Strachan
Author: Matthew R. Begley
Author: David Finkler
Author: Lindsay A. L. Bazydlo
Author: N. Scott Barker
Author: Doris M. Haverstick
Author: Marcel Utz ORCID iD
Author: James P. Landers

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