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Expression of biomineralization-related ion transport genes in Emiliania huxleyi

Expression of biomineralization-related ion transport genes in Emiliania huxleyi
Expression of biomineralization-related ion transport genes in Emiliania huxleyi
Biomineralization in the marine phytoplankton Emiliania huxleyi is a stringently controlled intracellular process. The molecular basis of coccolith production is still relatively unknown although its importance in global biogeochemical cycles and varying sensitivity to increased pCO2 levels has been well documented. This study looks into the role of several candidate Ca2+, H+ and inorganic carbon transport genes in E. huxleyi, using quantitative reverse transcriptase PCR. Differential gene expression analysis was investigated in two isogenic pairs of calcifying and non-calcifying strains of E. huxleyi and cultures grown at various Ca2+ concentrations to alter calcite production. We show that calcification correlated to the consistent upregulation of a putative HCO3- transporter belonging to the solute carrier 4 (SLC4) family, a Ca2+/H+ exchanger belonging to the CAX family of exchangers and a vacuolar H+-ATPase. We also show that the coccolith-associated protein, GPA is downregulated in calcifying cells. The data provide strong evidence that these genes play key roles in E. huxleyi biomineralization. Based on the gene expression data and the current literature a working model for biomineralization-related ion transport in coccolithophores is presented.
1462-2920
3250-3265
Mackinder, Luke
d1fafc3e-6ba1-4a27-bed1-1e55ce8ab2f2
Wheeler, Glen
13488538-691c-438f-88fe-e2d5e0d38278
Schroeder, Declan
c3e25db4-5af2-4792-8eda-bd3c94ad03ba
von Dassow, Peter
74ef5c44-af62-4509-949a-30b6616d05ff
Riebesell, Ulf
2218bcf4-b444-4a1a-b268-9875762de458
Brownlee, Colin
2af37c1c-b2bf-4832-8370-d9c35e7b3385
Mackinder, Luke
d1fafc3e-6ba1-4a27-bed1-1e55ce8ab2f2
Wheeler, Glen
13488538-691c-438f-88fe-e2d5e0d38278
Schroeder, Declan
c3e25db4-5af2-4792-8eda-bd3c94ad03ba
von Dassow, Peter
74ef5c44-af62-4509-949a-30b6616d05ff
Riebesell, Ulf
2218bcf4-b444-4a1a-b268-9875762de458
Brownlee, Colin
2af37c1c-b2bf-4832-8370-d9c35e7b3385

Mackinder, Luke, Wheeler, Glen, Schroeder, Declan, von Dassow, Peter, Riebesell, Ulf and Brownlee, Colin (2011) Expression of biomineralization-related ion transport genes in Emiliania huxleyi. Environmental Microbiology, 13 (12), 3250-3265. (doi:10.1111/j.1462-2920.2011.02561.x).

Record type: Article

Abstract

Biomineralization in the marine phytoplankton Emiliania huxleyi is a stringently controlled intracellular process. The molecular basis of coccolith production is still relatively unknown although its importance in global biogeochemical cycles and varying sensitivity to increased pCO2 levels has been well documented. This study looks into the role of several candidate Ca2+, H+ and inorganic carbon transport genes in E. huxleyi, using quantitative reverse transcriptase PCR. Differential gene expression analysis was investigated in two isogenic pairs of calcifying and non-calcifying strains of E. huxleyi and cultures grown at various Ca2+ concentrations to alter calcite production. We show that calcification correlated to the consistent upregulation of a putative HCO3- transporter belonging to the solute carrier 4 (SLC4) family, a Ca2+/H+ exchanger belonging to the CAX family of exchangers and a vacuolar H+-ATPase. We also show that the coccolith-associated protein, GPA is downregulated in calcifying cells. The data provide strong evidence that these genes play key roles in E. huxleyi biomineralization. Based on the gene expression data and the current literature a working model for biomineralization-related ion transport in coccolithophores is presented.

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e-pub ahead of print date: 8 September 2011
Published date: December 2011
Organisations: Ocean and Earth Science

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Local EPrints ID: 340272
URI: https://eprints.soton.ac.uk/id/eprint/340272
ISSN: 1462-2920
PURE UUID: 5a5a94b1-f0f9-41bd-a474-fa0267f1e94e

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Date deposited: 18 Jun 2012 13:46
Last modified: 11 Nov 2019 21:02

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