The University of Southampton
University of Southampton Institutional Repository

Rapid spatiotemporal patterning of cytosolic Ca2+ underlies flagellar excision in Chlamydomonas reinhardtii

Rapid spatiotemporal patterning of cytosolic Ca2+ underlies flagellar excision in Chlamydomonas reinhardtii
Rapid spatiotemporal patterning of cytosolic Ca2+ underlies flagellar excision in Chlamydomonas reinhardtii
Ca2+-dependent signalling processes are implicated in many aspects of flagella function in the green alga, Chlamydomonas. In this study, we examine the spatiotemporal dynamics of cytosolic Ca2+ ([Ca2+]cyt) in single Chlamydomonas cells during the process of flagellar excision, using biolistically loaded calcium-responsive dyes. Acid-induced deflagellation occurred in parallel with a single transient elevation in whole-cell [Ca2+]cyt, which was absent in the acid deflagellation-deficient adf1 mutant. Deflagellation could also be induced by elevated external Ca2+ ([Ca2+]ext), which promoted very rapid spiking of [Ca2+]cyt across the whole cell and in the flagella. We also detected very rapid apically localised Ca2+ signalling events with an approximate duration of 500 msec. Ninety-seven per cent of deflagellation events coincided with a rapid elevation in [Ca2+]cyt in the apical region of the cell, either in the form of a whole cell or an apically localised increase, indicating that [Ca2+]cyt elevations in the apical region play an underlying role in deflagellation. Our data indicate that elevated [Ca2+]ext acts to disrupt Ca2+ homeostasis which induces deflagellation by both Adf1-dependent and Adf1-independent mechanisms. Elevated [Ca2+]ext also results in further [Ca2+]cyt elevations after the main period of whole cell spiking which are very strongly associated with deflagellation, exhibit a high degree of apical localisation and are largely absent in the adf1 mutant. We propose that these later elevations may act as specific signals for deflagellation.
0960-7412
401-413
Wheeler, Glen L.
80ee477b-ceb3-4051-923c-399098bb746a
Joint, Ian
a0b88830-c422-4390-b652-2602d59e5813
Brownlee, Colin
2af37c1c-b2bf-4832-8370-d9c35e7b3385
Wheeler, Glen L.
80ee477b-ceb3-4051-923c-399098bb746a
Joint, Ian
a0b88830-c422-4390-b652-2602d59e5813
Brownlee, Colin
2af37c1c-b2bf-4832-8370-d9c35e7b3385

Wheeler, Glen L., Joint, Ian and Brownlee, Colin (2007) Rapid spatiotemporal patterning of cytosolic Ca2+ underlies flagellar excision in Chlamydomonas reinhardtii. The Plant Journal, 53 (3), 401-413. (doi:10.1111/j.1365-313X.2007.03349.x).

Record type: Article

Abstract

Ca2+-dependent signalling processes are implicated in many aspects of flagella function in the green alga, Chlamydomonas. In this study, we examine the spatiotemporal dynamics of cytosolic Ca2+ ([Ca2+]cyt) in single Chlamydomonas cells during the process of flagellar excision, using biolistically loaded calcium-responsive dyes. Acid-induced deflagellation occurred in parallel with a single transient elevation in whole-cell [Ca2+]cyt, which was absent in the acid deflagellation-deficient adf1 mutant. Deflagellation could also be induced by elevated external Ca2+ ([Ca2+]ext), which promoted very rapid spiking of [Ca2+]cyt across the whole cell and in the flagella. We also detected very rapid apically localised Ca2+ signalling events with an approximate duration of 500 msec. Ninety-seven per cent of deflagellation events coincided with a rapid elevation in [Ca2+]cyt in the apical region of the cell, either in the form of a whole cell or an apically localised increase, indicating that [Ca2+]cyt elevations in the apical region play an underlying role in deflagellation. Our data indicate that elevated [Ca2+]ext acts to disrupt Ca2+ homeostasis which induces deflagellation by both Adf1-dependent and Adf1-independent mechanisms. Elevated [Ca2+]ext also results in further [Ca2+]cyt elevations after the main period of whole cell spiking which are very strongly associated with deflagellation, exhibit a high degree of apical localisation and are largely absent in the adf1 mutant. We propose that these later elevations may act as specific signals for deflagellation.

Text
abstract - Version of Record
Restricted to Repository staff only
Request a copy

More information

Published date: 23 October 2007
Organisations: Ocean and Earth Science

Identifiers

Local EPrints ID: 340280
URI: http://eprints.soton.ac.uk/id/eprint/340280
ISSN: 0960-7412
PURE UUID: 1abe49a1-0d7f-4069-a361-f1fcbb7b844a

Catalogue record

Date deposited: 18 Jun 2012 14:06
Last modified: 08 Jan 2022 11:58

Export record

Altmetrics

Contributors

Author: Glen L. Wheeler
Author: Ian Joint
Author: Colin Brownlee

Download statistics

Downloads from ePrints over the past year. Other digital versions may also be available to download e.g. from the publisher's website.

View more statistics

Atom RSS 1.0 RSS 2.0

Contact ePrints Soton: eprints@soton.ac.uk

ePrints Soton supports OAI 2.0 with a base URL of http://eprints.soton.ac.uk/cgi/oai2

This repository has been built using EPrints software, developed at the University of Southampton, but available to everyone to use.

We use cookies to ensure that we give you the best experience on our website. If you continue without changing your settings, we will assume that you are happy to receive cookies on the University of Southampton website.

×