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Calmodulin-dependent protein kinase gamma 3 (CamKIIgamma3) mediates the cell cycle resumption of metaphase II eggs in mouse

Calmodulin-dependent protein kinase gamma 3 (CamKIIgamma3) mediates the cell cycle resumption of metaphase II eggs in mouse
Calmodulin-dependent protein kinase gamma 3 (CamKIIgamma3) mediates the cell cycle resumption of metaphase II eggs in mouse
Mature mammalian eggs are ovulated arrested at meiotic metaphase II. Sperm break this arrest by an oscillatory Ca(2+) signal that is necessary and sufficient for the two immediate events of egg activation: cell cycle resumption and cortical granule release. Previous work has suggested that cell cycle resumption, but not cortical granule release, is mediated by calmodulin-dependent protein kinase II (CamKII). Here we find that mouse eggs contain detectable levels of only one CamKII isoform, gamma 3. Antisense morpholino knockdown of CamKIIgamma3 during oocyte maturation produces metaphase II eggs that are insensitive to parthenogenetic activation by Ca(2+) stimulation and insemination. The effect is specific to this morpholino, as a 5-base-mismatch morpholino is without effect, and is rescued by CamKIIgamma3 or constitutively active CamKII cRNAs. Although CamKII-morpholino-treated eggs fail to exit metaphase II arrest, cortical granule exocytosis is not blocked. Therefore, CamKIIgamma3 plays a necessary and sufficient role in transducing the oscillatory Ca(2+) signal into cell cycle resumption, but not into cortical granule release.
calcium, cell cycle, fertilization, mouse, CamKII (Camk2)
1477-9129
4077-4081
Chang, HY
10ef9357-7f7b-432f-86d3-33c03f348980
Minahan, K
f611d3dd-baba-413e-ba1c-2511f2f3d90a
Merriman, JA
252e23f3-b63f-46ed-9904-7986642b4afa
Jones, KT
73e8e2b5-cd67-4691-b1a9-4e7bc9066af4
Chang, HY
10ef9357-7f7b-432f-86d3-33c03f348980
Minahan, K
f611d3dd-baba-413e-ba1c-2511f2f3d90a
Merriman, JA
252e23f3-b63f-46ed-9904-7986642b4afa
Jones, KT
73e8e2b5-cd67-4691-b1a9-4e7bc9066af4

Chang, HY, Minahan, K, Merriman, JA and Jones, KT (2009) Calmodulin-dependent protein kinase gamma 3 (CamKIIgamma3) mediates the cell cycle resumption of metaphase II eggs in mouse. Development, 136 (24), 4077-4081. (doi:10.1242/dev.042143). (PMID:19906843)

Record type: Article

Abstract

Mature mammalian eggs are ovulated arrested at meiotic metaphase II. Sperm break this arrest by an oscillatory Ca(2+) signal that is necessary and sufficient for the two immediate events of egg activation: cell cycle resumption and cortical granule release. Previous work has suggested that cell cycle resumption, but not cortical granule release, is mediated by calmodulin-dependent protein kinase II (CamKII). Here we find that mouse eggs contain detectable levels of only one CamKII isoform, gamma 3. Antisense morpholino knockdown of CamKIIgamma3 during oocyte maturation produces metaphase II eggs that are insensitive to parthenogenetic activation by Ca(2+) stimulation and insemination. The effect is specific to this morpholino, as a 5-base-mismatch morpholino is without effect, and is rescued by CamKIIgamma3 or constitutively active CamKII cRNAs. Although CamKII-morpholino-treated eggs fail to exit metaphase II arrest, cortical granule exocytosis is not blocked. Therefore, CamKIIgamma3 plays a necessary and sufficient role in transducing the oscillatory Ca(2+) signal into cell cycle resumption, but not into cortical granule release.

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More information

e-pub ahead of print date: November 2009
Published date: December 2009
Keywords: calcium, cell cycle, fertilization, mouse, CamKII (Camk2)
Organisations: Centre for Biological Sciences

Identifiers

Local EPrints ID: 340339
URI: https://eprints.soton.ac.uk/id/eprint/340339
ISSN: 1477-9129
PURE UUID: c7a1aaa1-e3d0-4460-aebd-1b9a454fdcc5

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Date deposited: 19 Jun 2012 13:31
Last modified: 18 Jul 2017 05:45

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