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Adult Circadian behavior in Drosophila requires developmental expression of cycle, but not period

Adult Circadian behavior in Drosophila requires developmental expression of cycle, but not period
Adult Circadian behavior in Drosophila requires developmental expression of cycle, but not period
Circadian clocks have evolved as internal time keeping mechanisms that allow anticipation of daily environmental changes and organization of a daily program of physiological and behavioral rhythms. To better examine the mechanisms underlying circadian clocks in animals and to ask whether clock gene expression and function during development affected subsequent daily time keeping in the adult, we used the genetic tools available in Drosophila to conditionally manipulate the function of the CYCLE component of the positive regulator CLOCK/CYCLE (CLK/CYC) or its negative feedback inhibitor PERIOD (PER). Differential manipulation of clock function during development and in adulthood indicated that there is no developmental requirement for either a running clock mechanism or expression of per. However, conditional suppression of CLK/CYC activity either via per over-expression or cyc depletion during metamorphosis resulted in persistent arrhythmic behavior in the adult. Two distinct mechanisms were identified that may contribute to this developmental function of CLK/CYC and both involve the ventral lateral clock neurons (LNvs) that are crucial to circadian control of locomotor behavior: (1) selective depletion of cyc expression in the LNvs resulted in abnormal peptidergic small-LNv dorsal projections, and (2) PER expression rhythms in the adult LNvs appeared to be affected by developmental inhibition of CLK/CYC activity. Given the conservation of clock genes and circuits among animals, this study provides a rationale for investigating a possible similar developmental role of the homologous mammalian CLOCK/BMAL1 complex.
1553-7390
e1002167-[19pp]
Goda, Tadahiro
e630d6d8-4e4b-48e5-85ee-5a4f0e56c0d4
Mirowska, Karolina
2f037b60-b46a-4cad-ad93-4e6f8f520fb2
Currie, Jake
eadec14d-818f-49e8-85b8-2b0616231002
Kim, Min-Ho
70ecaf2b-6d10-44ce-b7bb-708827a51dc1
Rao, Neethi Varadaraja
a6e9d429-a6a0-4c69-8575-f22809b8b02a
Bonilla, Gloribel
11ead339-cd29-4cab-aef0-2e3db3ad7ac6
Wijnen, Herman
67e9bc5d-de6e-44ec-b4c2-50b67c5bc79d
Goda, Tadahiro
e630d6d8-4e4b-48e5-85ee-5a4f0e56c0d4
Mirowska, Karolina
2f037b60-b46a-4cad-ad93-4e6f8f520fb2
Currie, Jake
eadec14d-818f-49e8-85b8-2b0616231002
Kim, Min-Ho
70ecaf2b-6d10-44ce-b7bb-708827a51dc1
Rao, Neethi Varadaraja
a6e9d429-a6a0-4c69-8575-f22809b8b02a
Bonilla, Gloribel
11ead339-cd29-4cab-aef0-2e3db3ad7ac6
Wijnen, Herman
67e9bc5d-de6e-44ec-b4c2-50b67c5bc79d

Goda, Tadahiro, Mirowska, Karolina, Currie, Jake, Kim, Min-Ho, Rao, Neethi Varadaraja, Bonilla, Gloribel and Wijnen, Herman (2011) Adult Circadian behavior in Drosophila requires developmental expression of cycle, but not period. PLoS Genetics, 7 (7), e1002167-[19pp]. (doi:10.1371/journal.pgen.1002167). (PMID:21750685)

Record type: Article

Abstract

Circadian clocks have evolved as internal time keeping mechanisms that allow anticipation of daily environmental changes and organization of a daily program of physiological and behavioral rhythms. To better examine the mechanisms underlying circadian clocks in animals and to ask whether clock gene expression and function during development affected subsequent daily time keeping in the adult, we used the genetic tools available in Drosophila to conditionally manipulate the function of the CYCLE component of the positive regulator CLOCK/CYCLE (CLK/CYC) or its negative feedback inhibitor PERIOD (PER). Differential manipulation of clock function during development and in adulthood indicated that there is no developmental requirement for either a running clock mechanism or expression of per. However, conditional suppression of CLK/CYC activity either via per over-expression or cyc depletion during metamorphosis resulted in persistent arrhythmic behavior in the adult. Two distinct mechanisms were identified that may contribute to this developmental function of CLK/CYC and both involve the ventral lateral clock neurons (LNvs) that are crucial to circadian control of locomotor behavior: (1) selective depletion of cyc expression in the LNvs resulted in abnormal peptidergic small-LNv dorsal projections, and (2) PER expression rhythms in the adult LNvs appeared to be affected by developmental inhibition of CLK/CYC activity. Given the conservation of clock genes and circuits among animals, this study provides a rationale for investigating a possible similar developmental role of the homologous mammalian CLOCK/BMAL1 complex.

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e-pub ahead of print date: 7 July 2011
Published date: July 2011
Organisations: Centre for Biological Sciences

Identifiers

Local EPrints ID: 340366
URI: http://eprints.soton.ac.uk/id/eprint/340366
ISSN: 1553-7390
PURE UUID: 13500771-0a41-43c5-8cd7-31601e7c0224
ORCID for Herman Wijnen: ORCID iD orcid.org/0000-0002-8710-5176

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Date deposited: 19 Jun 2012 15:34
Last modified: 17 Dec 2019 01:38

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