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Fluorescence/luminescence circadian imaging of complex tissues at single-cell resolution

Fluorescence/luminescence circadian imaging of complex tissues at single-cell resolution
Fluorescence/luminescence circadian imaging of complex tissues at single-cell resolution
The use of luciferase reporter genes together with luminescence detection has enabled high frequency monitoring of molecular circadian clock function in living tissues. With the help of an intensified CCD camera combined with an inverted epifluorescence microscope, the authors have established a new imaging strategy that makes use of transgenic cell type-specific expression of fluorescent proteins to identify cells of interest for subsequent circadian luminescence recording at single-cell resolution.
circadian clock, drosophila, imaging, luciferase, luminescence, fluorescence, gene expression
0748-7304
228-232
Sellix, Michael T.
84b0806d-ced4-404f-a3cb-c80a49a2ce90
Currie, Jake
eadec14d-818f-49e8-85b8-2b0616231002
Menaker, Michael
bf224455-45fc-4d1a-b9e9-e7d00a657303
Wijnen, Herman
67e9bc5d-de6e-44ec-b4c2-50b67c5bc79d
Sellix, Michael T.
84b0806d-ced4-404f-a3cb-c80a49a2ce90
Currie, Jake
eadec14d-818f-49e8-85b8-2b0616231002
Menaker, Michael
bf224455-45fc-4d1a-b9e9-e7d00a657303
Wijnen, Herman
67e9bc5d-de6e-44ec-b4c2-50b67c5bc79d

Sellix, Michael T., Currie, Jake, Menaker, Michael and Wijnen, Herman (2010) Fluorescence/luminescence circadian imaging of complex tissues at single-cell resolution. Journal of Biological Rhythms, 25 (3), 228-232. (doi:10.1177/0748730410368016). (PMID:20484694)

Record type: Article

Abstract

The use of luciferase reporter genes together with luminescence detection has enabled high frequency monitoring of molecular circadian clock function in living tissues. With the help of an intensified CCD camera combined with an inverted epifluorescence microscope, the authors have established a new imaging strategy that makes use of transgenic cell type-specific expression of fluorescent proteins to identify cells of interest for subsequent circadian luminescence recording at single-cell resolution.

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More information

Published date: June 2010
Additional Information: To whom all correspondence should be addressed: Herman Wijnen, University of Virginia, Department of Biology, 264 Gilmer Hall.
Keywords: circadian clock, drosophila, imaging, luciferase, luminescence, fluorescence, gene expression
Organisations: Centre for Biological Sciences

Identifiers

Local EPrints ID: 340367
URI: http://eprints.soton.ac.uk/id/eprint/340367
ISSN: 0748-7304
PURE UUID: d27a1a8f-f0df-4140-a3f9-ef423bb27d6c
ORCID for Herman Wijnen: ORCID iD orcid.org/0000-0002-8710-5176

Catalogue record

Date deposited: 19 Jun 2012 15:42
Last modified: 29 Aug 2019 00:35

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